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Literature summary for 2.6.1.1 extracted from

  • Yagi, T.; Sako, M.; Moriuti, S.; Shounaka, M.; Masaki, K.; Yamamoto, S.
    Purification and characterization of aspartate aminotransferase isoenzymes from rice bran (1993), Biosci. Biotechnol. Biochem., 57, 2074-2080.
No PubMed abstract available
Search for more literature for 2.6.1.1

Crystallization (Commentary)

Crystallization (Comment) Organism
concentrated isoenzyme AAT-1 solution, 10 mM Tris-HCl, pH 8.0, 1 mM 2-oxoglutarate, 2 mM KOH, 0.01 pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, polyethylene glycol 6000 8% w/v, 4°C, 6 days Oryza sativa
concentrated isoenzyme AAT-2 solution, 10 mM potassium phosphate, pH 7.0, 1 mM 2-oxoglutarate, 2 mM KOH, 0.001 mM pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, dialysis against distilled water at 4°C for 3 days Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 oxaloacetate isoenzyme AAT-1 Oryza sativa
0.048
-
 oxaloacetate isoenzyme AAT-2 Oryza sativa
0.105
-
 2-oxoglutarate isoenzyme AAT-2, pH 7.8, 37°C Oryza sativa
0.11
-
 2-oxoglutarate isoenzyme AAT-1, pH 7.8, 37°C Oryza sativa
2.4
-
 L-aspartate isoenzyme AAT-1, pH 7.8, 37°C Oryza sativa
3.7
-
 L-aspartate isoenzyme AAT-2, pH 7.8, 37°C Oryza sativa
5
-
 L-tryptophan L-glutamate, isoenzyme AAT-1 Oryza sativa
13.2
-
 L-glutamate isoenzyme AAT-2 Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 2 * 44000, isozyme AAT-1, SDS-PAGE Oryza sativa
47000
-
 2 * 47000, AAT-2, SDS-PAGE Oryza sativa
88000
-
 gel filtration Oryza sativa
88000
-
 isozyme AAT-1 Oryza sativa
94000
-
 isozyme AAT-2, gel filtration Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Oryza sativa
-
 oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 cv. Koganemasari
-
Oryza sativa
-
 2 isoenzymes: AAT-1, AAT-2
-

Purification (Commentary)

Purification (Comment) Organism
2 isoenzymes: AAT-1, AAT-2 Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Oryza sativa
-
root
-
 Oryza sativa
-
seed coat bran Oryza sativa
-
stem
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Oryza sativa
390
-
 purified isozyme AAT-1 Oryza sativa
450
-
 purified isozyme AAT-2 Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
 Oryza sativa oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Oryza sativa oxaloacetate + L-glutamate
-
 r
L-cysteine sulfinic acid + 2-oxoglutarate 63% of the activity with aspartate Oryza sativa 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ?
L-glutamate + 2-oxoglutarate L-glutamate is best amino acid donor Oryza sativa 2-oxoglutarate + L-glutamate
-
 ?
L-phenylalanine + 2-oxoglutarate
-
 Oryza sativa 2-oxo-3-phenylpropionic acid + L-glutamate i.e. phenylpyruvate ?
L-tryptophan + 2-oxoglutarate
-
 Oryza sativa 3-indole-2-oxopropionic acid + L-glutamate
-
 ?
additional information substrate specificity Oryza sativa ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 44000, isozyme AAT-1, SDS-PAGE Oryza sativa
dimer 2 * 47000, AAT-2, SDS-PAGE Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 forward reaction, isoenzymes AAT-1 and AAT-2 Oryza sativa
8 8.5 reverse reaction, isoenzyme AAT-1 Oryza sativa
8 8.8 reverse reaction, isoenzyme AAT-2 Oryza sativa

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Oryza sativa
pyridoxal 5'-phosphate isozyme AAT-1: 0.87 mol per mol of subunit, isozyme AAT-2: 0.93 mol per mol of subunit Oryza sativa

pI Value

Organism Comment pI Value Maximum pI Value
Oryza sativa isozyme AAT-2
-
 5
Oryza sativa isozyme AAT-1
-
 6.5