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Literature summary for 2.5.1.78 extracted from

  • Morgunova, E.; Saller, S.; Haase, I.; Cushman, M.; Bacher, A.; Fischer, M.; Ladenstein, R.
    Lumazine synthase from Candida albicans as an anti-fungal target enzyme: structural and biochemical basis for drug design (2007), J. Biol. Chem., 282, 17231-17241.
    View publication on PubMed
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Application

Application Comment Organism
medicine the fact that the enzymes of the riboflavin biosynthesis pathway are not present in the human or animal host makes them potential targets for anti-infective agents Candida albicans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Candida albicans

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallized in sitting drops by vapor diffusion. The crystal structure of lumazine synthase from Candida albicans is solved by molecular replacement and refined at 2.5 A resolution. The results of crystallographic investigations and sedimentation equilibrium experiments clearly indicate the presence of pentameric assemblies of the enzyme either in crystals or in solution Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
1,3,7-trihydro-9-D-ribityl-2,4,8-purinetrione association constants and thermodynamic parameters of binding of different inhibitors to lumazine synthase Candida albicans
3-(1,3-dihydro-9-D-ribityl-2,4,8-purinetrione-7-yl)propane 1-phosphate association constants and thermodynamic parameters of binding of different inhibitors to lumazine synthase Candida albicans
4-(6,7(5H,8H)-dioxo-8-D-ribityllumazine-5-yl)butane 1-phosphate association constants and thermodynamic parameters of binding of different inhibitors to lumazine synthase Candida albicans
4-(6-chloro-2,4-dioxo-1,2,3,4-tetrahydropyrimidine-5-yl)-butyl phosphate association constants and thermodynamic parameters of binding of different inhibitors to lumazine synthase Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate Candida albicans the enzyme is involved in riboflavin biosynthesis in many plants and microorganisms 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Candida albicans
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-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Candida albicans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.166
-
-
 Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Candida albicans 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate the enzyme is involved in riboflavin biosynthesis in many plants and microorganisms Candida albicans 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?