Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.78 extracted from

  • Koch, M.; Breithaupt, C.; Gerhardt, S.; Haase, I.; Weber, S.; Cushman, M.; Huber, R.; Bacher, A.; Fischer, M.
    Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase (2004), Eur. J. Biochem., 271, 3208-3214.
    View publication on PubMed
Search for more literature for 2.5.1.78

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals are grown at 18°C by the sitting drop vapor diffusion method. The W27Y mutant protein in complex with riboflavin, the substrate analogue 5-nitroso-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, and the product analogue 6-carboxyethyl-7-oxo-8-ribityllumazine, are determined by X-ray crystallography at resolutions of 2.7–2.8 A Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
W27Y whereas the indole system of W27 forms a coplanar pi-complex with riboflavin, the corresponding phenyl ring in the W27Y mutant establishes only peripheral contact with the heterocyclic ring system of the bound riboflavin Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
5-nitro-6-(D-ribitylamino)-2,4(1H,3H)-pyrimidinedione
-
 Schizosaccharomyces pombe
5-nitroso-6-(D-ribitylamino)-2,4(1H,3H)-pyrimidinedione
-
 Schizosaccharomyces pombe
6-carboxyethyl-7-oxo-8-ribityllumazine
-
 Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate Schizosaccharomyces pombe
-
 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Schizosaccharomyces pombe 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
6,7-dimethyl-8-ribityllumazine synthase
-
 Schizosaccharomyces pombe