Cloned (Comment) | Organism |
---|---|
expression of beta60 capsid | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
molecular modeling of inhibitors to the active site | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-[2,4,7-trioxo-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-1,2,3,4,7,8-hexahydropteridin-6-yl]butanoic acid | uncompetitive, comparison with inhibition of Escherichia coli riboflavin synthase | Bacillus subtilis | |
4-[2,4,7-trioxo-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-1,2,3,4,7,8-hexahydropteridin-6-yl]butyl dihydrogen phosphate | competitive, comparison with inhibition of Escherichia coli riboflavin synthase | Bacillus subtilis | |
4-[2,4,7-trioxo-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-1,2,3,4,7,8-hexahydropteridin-6-yl]propyl dihydrogen phosphate | uncompetitive, comparison with inhibition of Escherichia coli riboflavin synthase | Bacillus subtilis | |
5-(hexyl 6-dihydrogen phosphate)-6-([(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]amino)pyrimidine-2,4(1H,3H)-dione | mixed inhibition, comparison with inhibition of Escherichia coli riboflavin synthase | Bacillus subtilis | |
5-(pentyl 6-dihydrogen phosphate)-6-([(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]amino)pyrimidine-2,4(1H,3H)-dione | mixed inhibition, comparison with inhibition of Escherichia coli riboflavin synthase | Bacillus subtilis | |
6-methyl-7-methylidene-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-7,8-dihydropyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione | mixed inhibition, comparison with inhibition of Escherichia coli riboflavin synthase | Bacillus subtilis | |
9-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-7,9-dihydro-1H-purine-2,6,8(3H)-trione | comparison with inhibition of Escherichia coli riboflavin synthase | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
lumazine synthase/riboflavin synthase complex | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | - |
Bacillus subtilis | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
9-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-7,9-dihydro-1H-purine-2,6,8(3H)-trione | pH 7.5, 37°C, recombinant beta60 capsid | Bacillus subtilis | |
0.084 | - |
4-[2,4,7-trioxo-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-1,2,3,4,7,8-hexahydropteridin-6-yl]butanoic acid | pH 7.5, 37°C, recombinant beta60 capsid | Bacillus subtilis | |
0.094 | - |
6-methyl-7-methylidene-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-7,8-dihydropyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione | pH 7.5, 37°C, recombinant beta60 capsid | Bacillus subtilis | |
0.12 | - |
4-[2,4,7-trioxo-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-1,2,3,4,7,8-hexahydropteridin-6-yl]propyl dihydrogen phosphate | pH 7.5, 37°C, recombinant beta60 capsid | Bacillus subtilis | |
0.13 | - |
5-(hexyl 6-dihydrogen phosphate)-6-([(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]amino)pyrimidine-2,4(1H,3H)-dione | pH 7.5, 37°C, recombinant beta60 capsid | Bacillus subtilis | |
0.16 | - |
4-[2,4,7-trioxo-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-1,2,3,4,7,8-hexahydropteridin-6-yl]butyl dihydrogen phosphate | pH 7.5, 37°C, recombinant beta60 capsid | Bacillus subtilis | |
0.18 | - |
5-(pentyl 6-dihydrogen phosphate)-6-([(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]amino)pyrimidine-2,4(1H,3H)-dione | pH 7.5, 37°C, recombinant beta60 capsid | Bacillus subtilis |