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Literature summary for 2.5.1.7 extracted from

  • Shahab, M.; Verma, M.; Pathak, M.; Mitra, K.; Misra-Bhattacharya, S.
    Cloning, expression and characterization of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from Wolbachia endosymbiont of human lymphatic filarial parasite Brugia malayi (2014), PLoS ONE, 9, e99884.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3)pLysS cells Wolbachia sp.

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
 Wolbachia sp.
Fe2+
-
 Wolbachia sp.
fosfomycin about 50% residual activity at 50 mM Wolbachia sp.
Mn2+
-
 Wolbachia sp.
Ni2+
-
 Wolbachia sp.
phosphoenolpyruvate about 65% residual activity at 2.5 mM Wolbachia sp.

Metals/Ions

Metals/Ions Comment Organism Structure
K+ highest activity in the presence of K+ Wolbachia sp.
additional information not influenced by NH3+, Ca2+, Mg2+, and Cu2+ Wolbachia sp.
Na+
-
 Wolbachia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 x * 51000, SDS-PAGE Wolbachia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine Wolbachia sp.
-
 phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Wolbachia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chelating resin column chromatography, and gel filtration Wolbachia sp.

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine
-
 Wolbachia sp. phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, SDS-PAGE Wolbachia sp.

Synonyms

Synonyms Comment Organism
MurA
-
 Wolbachia sp.
UDP-N-acetylglucosamine enolpyruvyl transferase
-
 Wolbachia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Wolbachia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Wolbachia sp.