Protein Variants | Comment | Organism |
---|---|---|
C117D | the mutation shifts ithe optimum pH from 7.8 to 6.0. The mutant is not inhibited by 1 mM fosfomycin | Haemophilus influenzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosfomycin | more than 90% inhibition at 0.2 mM | Haemophilus influenzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
phosphoenolpyruvate | wild type enzyme, at pH 7.8 and 25°C | Haemophilus influenzae | |
0.031 | - |
UDP-N-acetyl-D-glucosamine | wild type enzyme, at pH 7.8 and 25°C | Haemophilus influenzae | |
0.15 | - |
phosphoenolpyruvate | mutant enzyme C117D, at pH 6.0 and 25°C | Haemophilus influenzae | |
0.16 | - |
UDP-N-acetyl-D-glucosamine | mutant enzyme C117D, at pH 6.0 and 25°C | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and gel filtration | Haemophilus influenzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.91 | - |
mutant enzyme C117D, at pH 6.0 and 25°C | Haemophilus influenzae |
4.7 | - |
wild type enzyme, at pH 7.8 and 25°C | Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Haemophilus influenzae | UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MurA | - |
Haemophilus influenzae |
UDP-N-acetylglucosamine enolpyruvyl transferase | - |
Haemophilus influenzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.68 | - |
phosphoenolpyruvate | mutant enzyme C117D, at pH 6.0 and 25°C | Haemophilus influenzae | |
0.68 | - |
UDP-N-acetyl-D-glucosamine | mutant enzyme C117D, at pH 6.0 and 25°C | Haemophilus influenzae | |
3.5 | - |
phosphoenolpyruvate | wild type enzyme, at pH 7.8 and 25°C | Haemophilus influenzae | |
3.5 | - |
UDP-N-acetyl-D-glucosamine | wild type enzyme, at pH 7.8 and 25°C | Haemophilus influenzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Haemophilus influenzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | recombinant enzyme, enzyme activity gradually decreases when the pH is below 5.5 or above 10.0 | Haemophilus influenzae |