Literature summary for 2.5.1.7 extracted from

Bensen, D.C.; Rodriguez, S.; Nix, J.; Cunningham, M.L.; Tari, L.W.
Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase) from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin (2012), Acta Crystallogr. Sect. F, 68, 382-385.

Search for more literature for 2.5.1.7

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 Star (DE3) cells	Aliivibrio fischeri

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with UDP-N-acetylglucosamine and fosfomycin, sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.1 M bis-Tris pH 6.5	Aliivibrio fischeri

Inhibitors

Inhibitors	Comment	Organism	Structure
fosfomycin	-	Aliivibrio fischeri

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45640	-	x * 45640, calculated from amino acid sequence	Aliivibrio fischeri

Organism

Organism	UniProt	Comment	Textmining
Aliivibrio fischeri	B5F9P4	-	-
Aliivibrio fischeri MJ11	B5F9P4	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography and Superdex 75 gel filtration	Aliivibrio fischeri

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine	-	Aliivibrio fischeri	UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + phosphate	-	?
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine	-	Aliivibrio fischeri MJ11	UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 45640, calculated from amino acid sequence	Aliivibrio fischeri

Synonyms

Synonyms	Comment	Organism
MurA	-	Aliivibrio fischeri
UDP-N-acetylglucosamine enolpyruvyl transferase	-	Aliivibrio fischeri

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Release 2023.1 (January 2023)