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Literature summary for 2.5.1.7 extracted from

  • Dube, S.; Nanda, K.; Rani, R.; Kaur, N.; Nagpal, J.; Upadhyay, D.; Cliffe, I.; Saini, K.; Purnapatre, K.
    UDP-N-acetylglucosamine enolpyruvyl transferase from Pseudomonas aeruginosa (2010), World J. Microbiol. Biotechnol., 26, 1623-1629.
    View publication on PubMed

Application

Application Comment Organism
drug development enzyme is an ideal target for the discovery of novel antibiotics against gram-negative pathogens as they have only one copy of murA gene in its genome Escherichia coli
drug development enzyme is an ideal target for the discovery of novel antibiotics against gram-negative pathogens as they have only one copy of murA gene in its genome Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
cloning and expression of MurA using pMAL expression system in frame with the fusion maltose binding protein in Escherichia coli BL21 Escherichia coli
cloning and expression of MurA using pMAL expression system in frame with the fusion maltose binding protein in Escherichia coli BL21 Pseudomonas aeruginosa

General Stability

General Stability Organism
no effect of 10% DMSO on the activity of enzyme Escherichia coli
no effect of 10% DMSO on the activity of enzyme Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
Co2+ metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM Escherichia coli
Cu2+ metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM Escherichia coli
Cu2+ metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM Pseudomonas aeruginosa
Fe2+ metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM Escherichia coli
Fe2+ metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM Pseudomonas aeruginosa
fosfomycin
-
Escherichia coli
fosfomycin
-
Pseudomonas aeruginosa
MnCl2 metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM Escherichia coli
MnCl2 metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM Pseudomonas aeruginosa
Zn2+ metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM Escherichia coli
Zn2+ metal ions do not enhance the activity of enzymes, activity is inhibited by 10 mM Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00045
-
phosphoenolpyruvate 0.125 mM Tris-HCl, pH 7.5 Pseudomonas aeruginosa
0.00084
-
phosphoenolpyruvate 0.125 mM Tris-HCl, pH 7.5 Escherichia coli
0.0178
-
UDP-N-acetyl-D-glucosamine 0.125 mM Tris-HCl, pH 7.5 Pseudomonas aeruginosa
0.036
-
UDP-N-acetyl-D-glucosamine 0.125 mM Tris-HCl, pH 7.5 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89000
-
as fusion protein with maltose binding protein, determined by SDS-PAGE Escherichia coli
89000
-
as fusion protein with maltose binding protein, determined by SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine Escherichia coli
-
phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
?
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine Pseudomonas aeruginosa
-
phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
MurA purification is done by using self packed amylose resin column Escherichia coli
MurA purification is done by using self packed amylose resin column Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
Escherichia coli phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
?
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
Pseudomonas aeruginosa phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
?

Synonyms

Synonyms Comment Organism
MurA
-
Escherichia coli
MurA
-
Pseudomonas aeruginosa
UDP-N-acetylglucosamine enolpyruvyl transferase
-
Escherichia coli
UDP-N-acetylglucosamine enolpyruvyl transferase
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Escherichia coli
37
-
-
Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
UDP-N-acetyl-D-glucosamine the calculated value for UDP is 40 times higher than that of ADP Escherichia coli
2.4
-
UDP-N-acetyl-D-glucosamine the calculated value for UDP is 40times higher than that of ADP Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli
7.5
-
assay at Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
6 9 enzyme shows activity at wide range of pH Escherichia coli
6 9 enzyme shows activity at wide range of pH Pseudomonas aeruginosa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00053
-
enzyme is pre-incubated with the inhibitor in the presence of 1 mM UDP-N-acetyl-D-glucosamine, reaction is started by addition of phosphoenolpyruvate Pseudomonas aeruginosa fosfomycin
0.00077
-
enzyme is pre-incubated with the inhibitor in the presence of 1 mM UDP-N-acetyl-D-glucosamine, reaction is started by addition of phosphoenolpyruvate Escherichia coli fosfomycin
0.0045
-
enzyme shows 5-7fold shift in IC50 for the inhibitor upon pre-incubation with the substrate UDP-N-acetyl-D-glucosamine Pseudomonas aeruginosa fosfomycin
0.0051
-
enzyme shows 5-7fold shift in IC50 for the inhibitor upon pre-incubation with the substrate UDP-N-acetyl-D-glucosamine Escherichia coli fosfomycin

General Information

General Information Comment Organism
malfunction gram-negative bacteria have only one copy of the murA gene, its deletion is lethal Escherichia coli
malfunction gram-negative bacteria have only one copy of the murA gene, its deletion is lethal Pseudomonas aeruginosa
physiological function enzyme catalyzes the first committed step of bacterial cell wall biosynthesis Escherichia coli
physiological function enzyme catalyzes the first committed step of bacterial cell wall biosynthesis Pseudomonas aeruginosa