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Literature summary for 2.5.1.7 extracted from
- Characterization and inhibition study of MurA enzyme by capillary electrophoresis (2002), J. Chromatogr. B, 766, 123-132.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | included in the assay reaction mixture | Escherichia coli |  |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | target for development of antibacterial agents | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fosfomycin | binding study by isothermal titration calorimetry; binds covalently to Cys115 | Escherichia coli | |
| PGE-553828 | competitive against UDP-GlcNAc; inhibition mechanism, kinetics | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Escherichia coli | |
| 0.0041 | - |
phosphoenolpyruvate | - |
Escherichia coli | |
| 0.0057 | - |
UDP-GlcNAc | - |
Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K-12 | - |
| Escherichia coli | - |
MurA | - |
| Escherichia coli MurA | - |
MurA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from overexpresssing strain | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Escherichia coli | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? | |
| phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Escherichia coli MurA | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.9 | - |
UDP-GlcNAc | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
PGE-553828 | - |
Escherichia coli |
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