Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UDP-N-acetylmuramyl-L-Ala-D-Glu-meso-alpha,epsilon-diaminopimelic acid | inhibitor binding site is distinct from active site | Enterobacter cloacae | |
uridine diphospho-N-acetylmuramyl-L-Ala-D-gamma-Glu-meso-alpha,epsilon-diaminopimelic-acid-D-Ala-D-Ala | inhibitor binding site is distinct from active site | Enterobacter cloacae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
gel filtration | Enterobacter cloacae |
41000 | - |
1 * 41000, SDS-PAGE | Enterobacter cloacae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | Enterobacter cloacae | enzyme catalyzes the first committed step in the biosynthesis of bacterial cell wall peptidoglycan | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Enterobacter cloacae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.9 | - |
- |
Enterobacter cloacae |
Storage Stability | Organism |
---|---|
-20°C, loss of activity after 1 week | Enterobacter cloacae |
4°C, in 80% ammonium sulfate, loss of activity after 1 week | Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Enterobacter cloacae | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate | ? | |
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | enzyme catalyzes the first committed step in the biosynthesis of bacterial cell wall peptidoglycan | Enterobacter cloacae | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Enterobacter cloacae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
uridine diphospho-N-acetylmuramyl-L-Ala-D-gamma-Glu-meso-alpha,epsilon-diaminopimelic-acid-D-Ala-D-Ala | - |
Enterobacter cloacae | |
4.9 | 6.6 | UDP-N-acetylmuramyl-L-Ala-D-Glu-meso-alpha,epsilon-diaminopimelic acid | - |
Enterobacter cloacae |