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Literature summary for 2.5.1.18 extracted from
- Molecular cloning and characterization of a sigma-class glutathione S-transferase from the freshwater mussel Hyriopsis cumingii (2015), Microbiol. Immunol., 59, 219-230.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)-pLysS cells | Hyriopsis cumingii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23300 | - |
- |
Hyriopsis cumingii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hyriopsis cumingii | A0A023I760 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel affinity resin column chromatography | Hyriopsis cumingii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adductor muscle | - |
Hyriopsis cumingii | - |
| foot | - |
Hyriopsis cumingii | - |
| gill | - |
Hyriopsis cumingii | - |
| gonad | - |
Hyriopsis cumingii | - |
| hemocyte | - |
Hyriopsis cumingii | - |
| hepatopancreas | strongest expression | Hyriopsis cumingii | - |
| intestine | - |
Hyriopsis cumingii | - |
| kidney | - |
Hyriopsis cumingii | - |
| mantle | - |
Hyriopsis cumingii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-chloro-2,4-dinitrobenzene + glutathione | - |
Hyriopsis cumingii | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23300, calculated from amino acid sequence | Hyriopsis cumingii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GSTS | - |
Hyriopsis cumingii |
| sigma-class glutathione S-transferase | - |
Hyriopsis cumingii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Hyriopsis cumingii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Hyriopsis cumingii |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Hyriopsis cumingii | the mRNA expression is significantly up-regulated in all tissues after stimulation with peptidoglycan and lipopolysaccharide. The expression of mRNA in kidney and foot is also significantly (about 38fold) up-regulated by microcystin-LR | up |
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Release 2023.1 (January 2023)
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