Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Alternaria brassicicola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTFuA1, at pH 7.5, temperature not specified in the publication | Alternaria brassicicola | |
0.45 | - |
glutathione | isoform GSTO1, at pH 7.5, temperature not specified in the publication | Alternaria brassicicola | |
0.5 | - |
glutathione | isoform GSTFuA1, at pH 7.5, temperature not specified in the publication | Alternaria brassicicola | |
0.87 | - |
glutathione | isoform Ure2pB1, at pH 7.5, temperature not specified in the publication | Alternaria brassicicola | |
1.33 | - |
glutathione | isoform GTT1.2, at pH 7.5, temperature not specified in the publication | Alternaria brassicicola | |
1.82 | - |
1-chloro-2,4-dinitrobenzene | isoform GTT1.2, at pH 7.5, temperature not specified in the publication | Alternaria brassicicola | |
5.01 | - |
1-chloro-2,4-dinitrobenzene | isoform Ure2pB1, at pH 7.5, temperature not specified in the publication | Alternaria brassicicola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alternaria brassicicola | - |
- |
- |
Alternaria brassicicola Abra43 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE cellulose column chromatography, and ACA44 gel filtration | Alternaria brassicicola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,4-dinitrobenzene + glutathione | - |
Alternaria brassicicola | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
1-chloro-2,4-dinitrobenzene + glutathione | - |
Alternaria brassicicola Abra43 | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
additional information | isoforms GTT1.2, GSTO1, and Ure2pB1 show no activity with allyl-isothiocyanate,, benzyl-isothiocyanate, or phenylethyl-isothiocyanate | Alternaria brassicicola | ? | - |
? | |
additional information | isoforms GTT1.2, GSTO1, and Ure2pB1 show no activity with allyl-isothiocyanate,, benzyl-isothiocyanate, or phenylethyl-isothiocyanate | Alternaria brassicicola Abra43 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione transferase | - |
Alternaria brassicicola |
GST | - |
Alternaria brassicicola |
GSTFuA1 | isoform | Alternaria brassicicola |
GSTO1 | isoform | Alternaria brassicicola |
GTT1.2 | isoform | Alternaria brassicicola |
Ure2pB1 | isoform | Alternaria brassicicola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
1-chloro-2,4-dinitrobenzene | isoform GTT1.2, at pH 7.5, temperature not specified in the publication | Alternaria brassicicola | |
1.5 | - |
1-chloro-2,4-dinitrobenzene | isoform Ure2pB1, at pH 7.5, temperature not specified in the publication | Alternaria brassicicola | |
38.18 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTFuA1, at pH 7.5, temperature not specified in the publication | Alternaria brassicicola |
General Information | Comment | Organism |
---|---|---|
physiological function | isoforms GTT1.2, GSTFuA1, and GSTO1 are essential for full aggressiveness of Alternaria brassicicola on host plant | Alternaria brassicicola |