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Literature summary for 2.5.1.18 extracted from

  • Calmes, B.; Morel-Rouhier, M.; Bataille-Simoneau, N.; Gelhaye, E.; Guillemette, T.; Simoneau, P.
    Characterization of glutathione transferases involved in the pathogenicity of Alternaria brassicicola (2015), BMC Microbiol., 15, 123.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Alternaria brassicicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 1-chloro-2,4-dinitrobenzene isoform GSTFuA1, at pH 7.5, temperature not specified in the publication Alternaria brassicicola
0.45
-
 glutathione isoform GSTO1, at pH 7.5, temperature not specified in the publication Alternaria brassicicola
0.5
-
 glutathione isoform GSTFuA1, at pH 7.5, temperature not specified in the publication Alternaria brassicicola
0.87
-
 glutathione isoform Ure2pB1, at pH 7.5, temperature not specified in the publication Alternaria brassicicola
1.33
-
 glutathione isoform GTT1.2, at pH 7.5, temperature not specified in the publication Alternaria brassicicola
1.82
-
 1-chloro-2,4-dinitrobenzene isoform GTT1.2, at pH 7.5, temperature not specified in the publication Alternaria brassicicola
5.01
-
 1-chloro-2,4-dinitrobenzene isoform Ure2pB1, at pH 7.5, temperature not specified in the publication Alternaria brassicicola

Organism

Organism UniProt Comment Textmining
Alternaria brassicicola
-
-
-
Alternaria brassicicola Abra43
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE cellulose column chromatography, and ACA44 gel filtration Alternaria brassicicola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione
-
 Alternaria brassicicola S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
1-chloro-2,4-dinitrobenzene + glutathione
-
 Alternaria brassicicola Abra43 S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
additional information isoforms GTT1.2, GSTO1, and Ure2pB1 show no activity with allyl-isothiocyanate,, benzyl-isothiocyanate, or phenylethyl-isothiocyanate Alternaria brassicicola ?
-
 ?
additional information isoforms GTT1.2, GSTO1, and Ure2pB1 show no activity with allyl-isothiocyanate,, benzyl-isothiocyanate, or phenylethyl-isothiocyanate Alternaria brassicicola Abra43 ?
-
 ?

Synonyms

Synonyms Comment Organism
glutathione transferase
-
 Alternaria brassicicola
GST
-
 Alternaria brassicicola
GSTFuA1 isoform Alternaria brassicicola
GSTO1 isoform Alternaria brassicicola
GTT1.2 isoform Alternaria brassicicola
Ure2pB1 isoform Alternaria brassicicola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 1-chloro-2,4-dinitrobenzene isoform GTT1.2, at pH 7.5, temperature not specified in the publication Alternaria brassicicola
1.5
-
 1-chloro-2,4-dinitrobenzene isoform Ure2pB1, at pH 7.5, temperature not specified in the publication Alternaria brassicicola
38.18
-
 1-chloro-2,4-dinitrobenzene isoform GSTFuA1, at pH 7.5, temperature not specified in the publication Alternaria brassicicola

General Information

General Information Comment Organism
physiological function isoforms GTT1.2, GSTFuA1, and GSTO1 are essential for full aggressiveness of Alternaria brassicicola on host plant Alternaria brassicicola