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Literature summary for 2.5.1.18 extracted from

  • Glisic, B.; Mihaljevic, I.; Popovic, M.; Zaja, R.; Loncar, J.; Fent, K.; Kovacevic, R.; Smital, T.
    Characterization of glutathione-S-transferases in zebrafish (Danio rerio) (2015), Aquat. Toxicol., 158, 50-62.
    View publication on PubMed
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Danio rerio

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 monochlorobimane isoform Gstt1a, at pH 6.5 and 25°C Danio rerio
0.05
-
 monochlorobimane isoform GstrI, at pH 6.5 and 25°C Danio rerio
0.11
-
 monochlorobimane isoform Gstm3, at pH 6.5 and 25°C Danio rerio
0.18
-
 monochlorobimane isoform Gstp2, at pH 6.5 and 25°C Danio rerio
0.2
-
 Ethacrynic acid isoform Gstp1, at pH 6.5 and 25°C Danio rerio
0.22
-
 monochlorobimane isoform Gstm1, at pH 6.5 and 25°C Danio rerio
0.24
-
 Ethacrynic acid isoform Gstp2, at pH 6.5 and 25°C Danio rerio
0.25
-
 monochlorobimane isoform Gstp1, at pH 6.5 and 25°C Danio rerio
0.27
-
 glutathione isoform Gstz1, at pH 6.5 and 25°C Danio rerio
0.27
-
 Dichloroacetic acid isoform Gstz1, at pH 6.5 and 25°C Danio rerio
0.28
-
 1-chloro-2,4-dinitrobenzene isoform Gstp2, at pH 6.5 and 25°C Danio rerio
0.32
-
 1-chloro-2,4-dinitrobenzene isoform Gstt1a, at pH 6.5 and 25°C Danio rerio
0.39
-
 1-chloro-2,4-dinitrobenzene isoform Gstp1, at pH 6.5 and 25°C Danio rerio
0.5
-
 glutathione isoform Gstr1, at pH 6.5 and 25°C Danio rerio
0.58
-
 glutathione isoform Gstm1, at pH 6.5 and 25°C Danio rerio
0.58
-
 1-chloro-2,4-dinitrobenzene isoform Gstr1, at pH 6.5 and 25°C Danio rerio
0.75
-
 glutathione isoform Gsta3, at pH 6.5 and 25°C Danio rerio
0.82
-
 glutathione isoform Gstp1, at pH 6.5 and 25°C Danio rerio
1.08
-
 dehydroascorbate isoform Gsto2, at pH 6.5 and 25°C Danio rerio
1.19
-
 glutathione isoform Gstp2, at pH 6.5 and 25°C Danio rerio
1.68
-
 1-chloro-2,4-dinitrobenzene isoform Gsta3, at pH 6.5 and 25°C Danio rerio
2.05
-
 glutathione isoform Gstm3, at pH 6.5 and 25°C Danio rerio
2.24
-
 1-chloro-2,4-dinitrobenzene isoform Gstm1, at pH 6.5 and 25°C Danio rerio
2.91
-
 glutathione isoform Gsto2, at pH 6.5 and 25°C Danio rerio
3.69
-
 1-chloro-2,4-dinitrobenzene isoform Gstm3, at pH 6.5 and 25°C Danio rerio
4.44
-
 glutathione isoform Gstta1, at pH 6.5 and 25°C Danio rerio

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Danio rerio 5829
-

Organism

Organism UniProt Comment Textmining
Danio rerio
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and gel filtration Danio rerio

Source Tissue

Source Tissue Comment Organism Textmining
brain high expression of isoforms Gstz1, Gstp, Gstm, Gstr1, Mgst3a, and Mgst3b Danio rerio
-
gill high expression of isoforms Gsto2, Gstp, Gstm, Gstr1, Mgst3a, and Mgst3b Danio rerio
-
intestine high expression of isoforms Gsta, Gstp, Gstm, Gstr1, Mgst3a, and Mgst3b Danio rerio
-
kidney high expression of isoforms Gstz1, Gstp, Gstm, Gstr1, Mgst3a, and Mgst3b Danio rerio
-
liver high expression of isoforms Gstt1a, Gstz1, Gstp, Gstr1, Mgst3a, and Mgst3b Danio rerio
-
ovary high expression of isoforms Gsto2, Gstp, Gstm, Gstr1, Mgst3a, and Mgst3b Danio rerio
-
testis high expression of isoforms Gsta, Gstp, Gstm, Gstr1, Mgst3a, and Mgst3b Danio rerio
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione
-
 Danio rerio S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
dehydroascorbate + glutathione substrate for isoform Gsto2 Danio rerio ?
-
 ?
dichloroacetic acid + glutathione substrate for isoform Gstz1 Danio rerio ?
-
 ?
ethacrynic acid + glutathione substrate for isoforms Gstp1 and Gstp2 Danio rerio ?
-
 ?
monochlorobimane + glutathione
-
 Danio rerio ?
-
 ?

Synonyms

Synonyms Comment Organism
glutathione-S-transferas
-
 Danio rerio
GST
-
 Danio rerio
Gsta isoform Danio rerio
Gstm isoform Danio rerio
GSTO2 isoform Danio rerio
GSTP isoform Danio rerio
Gstr1 isoform Danio rerio
Gstt1a isoform Danio rerio
Gstz1 isoform Danio rerio
Mgst3a isoform Danio rerio
Mgst3b isoform Danio rerio

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 Dichloroacetic acid isoform Gstz1, at pH 6.5 and 25°C Danio rerio
0.005
-
 glutathione isoform Gstz1, at pH 6.5 and 25°C Danio rerio
1.23
-
 Ethacrynic acid isoform Gstp2, at pH 6.5 and 25°C Danio rerio
2.24
-
 Ethacrynic acid isoform Gstp1, at pH 6.5 and 25°C Danio rerio
8.69
-
 glutathione isoform Gsto2, at pH 6.5 and 25°C Danio rerio
11.2
-
 glutathione isoform Gstta1, at pH 6.5 and 25°C Danio rerio
11.9
-
 dehydroascorbate isoform Gsto2, at pH 6.5 and 25°C Danio rerio
14.1
-
 glutathione isoform Gstp2, at pH 6.5 and 25°C Danio rerio
16.6
-
 glutathione isoform Gsta3, at pH 6.5 and 25°C Danio rerio
20.8
-
 glutathione isoform Gstr1, at pH 6.5 and 25°C Danio rerio
22.7
-
 glutathione isoform Gstm1, at pH 6.5 and 25°C Danio rerio
57.2
-
 glutathione isoform Gstm3, at pH 6.5 and 25°C Danio rerio
129
-
 glutathione isoform Gstp1, at pH 6.5 and 25°C Danio rerio