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Literature summary for 2.5.1.18 extracted from

  • Scian, M.; Le Trong, I.; Mazari, A.M.; Mannervik, B.; Atkins, W.M.; Stenkamp, R.E.
    Comparison of epsilon- and delta-class glutathione S-transferases: the crystal structures of the glutathione S-transferases DmGSTE6 and DmGSTE7 from Drosophila melanogaster (2015), Acta Crystallogr. Sect. D, 71, 2089-2098.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Drosophila melanogaster

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 20% (w/v) PEG 4000, 10% (v/v) 2-propanol, 2 mM GSH, 10 mM dithiothreitol Drosophila melanogaster
hanging drop vapor diffusion method, using 0.1 M Tris-HCl pH 7.5, 25% (w/v) PEG 4000, 2 mM GSH, and 10 mM dithiothreitol Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster A1ZB72
-
-
Drosophila melanogaster Q7JZM3
-
-

Purification (Commentary)

Purification (Comment) Organism
His60 Ni Superflow resin column chromatography and Superdex 200 gel filtration Drosophila melanogaster

Synonyms

Synonyms Comment Organism
GSTE6 isoform Drosophila melanogaster
GSTE7 isoform Drosophila melanogaster