Cloned (Comment) | Organism |
---|---|
isozyme GSTA1-1 expression in Escherichia coli strain BL21(DE3), expression of His-tagged isozymes GSTO1-1 and GSTT2-2 in Escherichia coli strain M15[pREP4] | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dieldrin | a potent reversible inhibitor toward hGSTA1-1, mixed-type inhibition, dieldrin binds specifically to the enzyme presumably at a position that partially overlaps with both the G- and H-site, a site distinct from binding of spiromesifen, binding structure overview | Homo sapiens | |
additional information | hGSTA1-1 is more susceptible to inhibition by pesticides than other isoenzymes. In silico docking analysis and kinetic inhibition studies, overview | Homo sapiens | |
spiromesifen | a potent reversible inhibitor toward hGSTA1-1, mixed-type inhibition, spiromesifen binds specifically to the enzyme presumably at a position that partially overlaps with both the G- and H-site, a site distinct from binding of dieldrin, binding structure overview | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme hGSTA1-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme GSTA1-1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, recombinant His-tagged isozymes GSTO1-1 and GSTT2-2 in Escherichia coli strain M15[pREP4] by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,4-dinitrobenzene + glutathione | - |
Homo sapiens | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
additional information | development of analytical quantification assays for pesticides dieldrin and spiromesifen in water with isozyme GSTA1-1, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hGSTA1-1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic inhibition studies with dieldrin and spiromesifen, overview | Homo sapiens | |
0.0001 | - |
dieldrin | versus 1-chloro-2,4-dinitrobenzene, pH 6.5, 37°C, recombinant wild-type GSTA1-1 | Homo sapiens | |
0.0004 | - |
spiromesifen | versus 1-chloro-2,4-dinitrobenzene, pH 6.5, 37°C, recombinant wild-type GSTA1-1 | Homo sapiens | |
0.0021 | - |
spiromesifen | versus glutathione, pH 6.5, 37°C, recombinant wild-type GSTA1-1 | Homo sapiens | |
0.0023 | - |
dieldrin | versus glutathione, pH 6.5, 37°C, recombinant wild-type GSTA1-1 | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0121 | - |
versus glutathione, pH 6.5, 37°C, recombinant wild-type GSTA1-1 | Homo sapiens | spiromesifen | |
0.0179 | - |
versus 1-chloro-2,4-dinitrobenzene, pH 6.5, 37°C, recombinant wild-type GSTA1-1 | Homo sapiens | dieldrin | |
0.0242 | - |
versus glutathione, pH 6.5, 20°C, recombinant wild-type GSTA1-1 | Homo sapiens | spiromesifen | |
0.0295 | - |
versus glutathione, pH 7.5, 37°C, recombinant wild-type GSTA1-1 | Homo sapiens | spiromesifen | |
0.0345 | - |
versus 1-chloro-2,4-dinitrobenzene, pH 6.5, 25°C, recombinant wild-type GSTA1-1 | Homo sapiens | dieldrin | |
0.0472 | - |
versus 1-chloro-2,4-dinitrobenzene, pH 7.0, 37°C, recombinant wild-type GSTA1-1 | Homo sapiens | dieldrin | |
0.0504 | - |
versus 1-chloro-2,4-dinitrobenzene, pH 7.5, 37°C, recombinant wild-type GSTA1-1 | Homo sapiens | dieldrin | |
0.062 | - |
versus glutathione, pH 7.0, 37°C, recombinant wild-type GSTA1-1 | Homo sapiens | spiromesifen | |
0.0795 | - |
versus glutathione, pH 6.5, 25°C, recombinant wild-type GSTA1-1 | Homo sapiens | spiromesifen | |
0.0983 | - |
versus 1-chloro-2,4-dinitrobenzene, pH 6.5, 20°C, recombinant wild-type GSTA1-1 | Homo sapiens | dieldrin |