Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lucilia cuprina | P42860 | isozyme GSTD1 or Delta or Theta class isozyme; Sigma and Delta GST isozymes | - |
Purification (Comment) | Organism |
---|---|
native Sigma and Delta class isozymes by gel filtration, glutathione affinity chromatography, and anion exchange chromatography, 103fold from pupae, 100fold from larvae, 51.7fold from egg (only Delta GST isozyme), and 52fold from adult | Lucilia cuprina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | analysis of GST activity and levels of the Sigma and Delta GSTs during development examined at the four developmental stages, egg, larvae, pupae and adult, tissue distribution, overview | Lucilia cuprina | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of isozymes from the different stages with other substrates, overview | Lucilia cuprina |
4.33 | - |
purified overall GST activity in adult, substrate 1-chloro-2,4-dinitrobenzene, pH and temperature not specified in the publication | Lucilia cuprina |
4.57 | - |
purified overall GST activity in eggs, substrate 1-chloro-2,4-dinitrobenzene, pH and temperature not specified in the publication | Lucilia cuprina |
6.17 | - |
purified overall GST activity in larvae, substrate 1-chloro-2,4-dinitrobenzene, pH and temperature not specified in the publication | Lucilia cuprina |
14.5 | - |
purified overall GST activity in pupae, substrate 1-chloro-2,4-dinitrobenzene, pH and temperature not specified in the publication | Lucilia cuprina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,4-dinitrobenzene + glutathione | best substrate | Lucilia cuprina | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
3,4-dichloronitrobenzene + glutathione | - |
Lucilia cuprina | ? | - |
? | |
4-nitrophenyl acetate + glutathione | - |
Lucilia cuprina | ? | - |
? | |
dehydroascorbic acid + glutathione | - |
Lucilia cuprina | ? | - |
? | |
trans-2-nonenal + glutathione | - |
Lucilia cuprina | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | peptide mapping of Sigma and Delta class isozymes using the sequences of GSTs from Drosophila melanogaster and Musca domestica, overview | Lucilia cuprina |
Synonyms | Comment | Organism |
---|---|---|
GSTD1 | - |
Lucilia cuprina |