Cloned (Comment) | Organism |
---|---|
isozyme GSTO, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional overexpression in Escherichia coli as soluble protein | Bombyx mori |
sequence comparisons and phylogenetic analysis | Bombyx mori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deltamethrin | 41% inhibition at 1 mM; 54% inhibition at 1 mM | Bombyx mori | |
fenitrothion | 50% inhibition at 1 mM; 56% inhibition at 1 mM | Bombyx mori | |
additional information | the enzyme is completely resistant against insecticides fenitrothion, deltamethrin, and permethrin, in vivo toxicity, overview | Bombyx mori | |
permethrin | 24% inhibition at 1 mM; 33% inhibition at 1 mM | Bombyx mori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Bombyx mori | |
0.006 | - |
4-hydroxynonenal | GSTO | Bombyx mori | |
0.14 | - |
4-hydroxynonenal | GSTD | Bombyx mori | |
0.48 | - |
1-chloro-2,4-dinitrobenzene | GSTD | Bombyx mori | |
0.67 | - |
1-chloro-2,4-dinitrobenzene | GSTO | Bombyx mori | |
1.6 | - |
1-chloro-2,4-dinitrobenzene | GSTS | Bombyx mori | |
2.1 | - |
4-hydroxynonenal | GSTS | Bombyx mori |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bombyx mori | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23338 | - |
x * 23338, GSTO, sequence calculation | Bombyx mori |
24225 | - |
x * 24225, GSTO, sequence calculation | Bombyx mori |
29806 | - |
x * 29806, GSTO, sequence calculation | Bombyx mori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | A8R5V3 | omega-GST | - |
Bombyx mori | Q5CCJ4 | sigma-GST | - |
Bombyx mori | Q60GK5 | delta-GST | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme GSTO from Escherichia coli to homogeneity by ammonium sulfate fractionation, anion exchange chromatography and gel filtration | Bombyx mori |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fat body | - |
Bombyx mori | - |
larva | fifth-instar larvae | Bombyx mori | - |
midgut | - |
Bombyx mori | - |
silk gland | low expression level | Bombyx mori | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,4-dinitrobenzene + glutathione | - |
Bombyx mori | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
4-hydroxynonenal + glutathione | - |
Bombyx mori | ? | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Bombyx mori | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
glutathione + 4-hydroxynonenal | a lipid peroxidation product | Bombyx mori | ? | - |
? | |
glutathione + 4-nitrophenyl acetate | - |
Bombyx mori | S-(4-nitrophenyl)glutathione + HCl | - |
? | |
glutathione + ethacrynic acid | - |
Bombyx mori | ? | - |
? | |
additional information | the enzyme shows high affinity for organophosphorus insecticide | Bombyx mori | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23338, GSTO, sequence calculation | Bombyx mori |
? | x * 24225, GSTO, sequence calculation | Bombyx mori |
? | x * 29806, GSTO, sequence calculation | Bombyx mori |
Synonyms | Comment | Organism |
---|---|---|
delta-class glutathione S-transferase | - |
Bombyx mori |
glutathione S-transferase | - |
Bombyx mori |
GSTD | - |
Bombyx mori |
GSTO | - |
Bombyx mori |
GSTS | - |
Bombyx mori |
omega-class glutathione S-transferase | - |
Bombyx mori |
sigma-class glutathione S-transferase | - |
Bombyx mori |
sigma-class GST | - |
Bombyx mori |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified GSTS, stable up to | Bombyx mori |
50 | - |
purified GSTD, stable up to | Bombyx mori |
50 | - |
purified GSTO, stable up to | Bombyx mori |
60 | - |
purified GSTO, 50% inhibitioin | Bombyx mori |
80 | - |
purified GSTO, inactivation | Bombyx mori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | GSTD | Bombyx mori |
7 | 8 | GSTO | Bombyx mori |
8 | - |
GSTS | Bombyx mori |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 10 | activity range, about 10% of maximal activity at pH 2.0 and pH 10.0 | Bombyx mori |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 12 | GSTO shows 75% of maximal activity at pH 2.0 and pH 12.0 after 24 h at 4°C | Bombyx mori |
4 | 11 | GSTS | Bombyx mori |
5 | 11 | GSTD | Bombyx mori |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bombyx mori | GSTS, sequence calculation | - |
5.79 |
Bombyx mori | GSTO, sequence calculation | - |
6.01 |
Bombyx mori | GSTD, sequence calculation | - |
8.35 |
General Information | Comment | Organism |
---|---|---|
physiological function | bmGSTO might be involved in the increase in level of insecticide resistance for lepidopteran insects | Bombyx mori |