Activating Compound | Comment | Organism | Structure |
---|---|---|---|
H2O2 | inhibits cytosolic GSTs and activates microsomal GSTs | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | inhibits cytosolic GST by about 65% and activates microsomal GST to about 175% | Rattus norvegicus | |
additional information | presence of superoxide dismutase and catalase reduces the activity of the microsomal GST isozyme, but only catalase can reduce the activity of the cytosolic GST | Rattus norvegicus | |
superoxide anion | generated by a Fe3+/ascorbate system, inhibits cytosolic GSTs and microsomal GSTs by 30% and 65%, respectively. Addition of 2 mM DTT fails to completely reverse microsomal GST inhibition elicited by Fe3+/ascorbate, recovering only about 10% of this activity, but it completely reverses the inhibitory effect of Fe3+/ascorbate on cytosolic GST activity | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
membrane | integral | Rattus norvegicus | 16020 | - |
microsome | - |
Rattus norvegicus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male sprague-dawley rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.05 | - |
microsomal isozyme in presence of catalase | Rattus norvegicus |
0.06 | - |
microsomal isozyme in presence of superoxide dismutase | Rattus norvegicus |
0.075 | - |
microsomal isozyme in presence of Fe3+/ascorbate | Rattus norvegicus |
0.139 | - |
microsomal isozyme | Rattus norvegicus |
0.692 | - |
cytosolic isozyme in presence of Fe3+/ascorbate | Rattus norvegicus |
0.702 | - |
cytosolic isozyme in presence of catalase | Rattus norvegicus |
0.984 | - |
cytosolic isozyme in presence of superoxide dismutase | Rattus norvegicus |
0.985 | - |
cytosolic isozyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Rattus norvegicus | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Rattus norvegicus |
GST | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |