Cloned (Comment) | Organism |
---|---|
GSTZ, library screening, cloning in Escherichia coli strain DH5alpha, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C19Y | random mutagenesis, inactive mutant | Arabidopsis thaliana |
K30E | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
K83E | random mutagenesis, the mutant shows 50% reduced activity compared to the wild-type enzyme | Arabidopsis thaliana |
L11P | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
L11P/I140T | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
L147F | random mutagenesis, the mutant shows 47.7% reduced activity compared to the wild-type enzyme | Arabidopsis thaliana |
L147P | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
L150P | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
L169R | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
L27P | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
L80P/N116S | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
additional information | mutant enzyme inactivation mechanism, molecular modeling, mutations resulting in inactivation of the enzyme are found in or near the binding pocket, whereas mutations resulting in partial inactivation are distant from both substrates, overview | Arabidopsis thaliana |
P61S | random mutagenesis, inactive mutant | Arabidopsis thaliana |
Q102R | random mutagenesis, the mutant shows 68.4% reduced activity compared to the wild-type enzyme | Arabidopsis thaliana |
Q112R | random mutagenesis, inactive mutant | Arabidopsis thaliana |
R22H/K83E | random mutagenesis, inactive mutant | Arabidopsis thaliana |
S105R/Q213R | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
S109P | random mutagenesis, inactive mutant | Arabidopsis thaliana |
S73P | random mutagenesis, inactive mutant | Arabidopsis thaliana |
S73X | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
W15R | random mutagenesis, inactive mutant | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dichloroacetic acid + glutathione | - |
Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | molecular modeling of the GSTZ substrate superimposed enzyme using the apoform crystal structure of AtGSTZ monomer | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
GSTZ | - |
Arabidopsis thaliana |
zeta class glutathione S-transferase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Arabidopsis thaliana |