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Literature summary for 2.5.1.18 extracted from

  • Larsson, A.K.; Shokeer, A.; Mannervik, B.
    Molecular evolution of theta-class glutathione transferase for enhanced activity with the anticancer drug 1,3-bis-(2-chloroethyl)-1-nitrosourea and other alkylating agents (2010), Arch. Biochem. Biophys., 497, 28-34.
    View publication on PubMed
Search for more literature for 2.5.1.18

Application

Application Comment Organism
medicine GSTs catalyzing inactivation of anticancer drugs may find clinical use in protecting sensitive normal tissues to toxic side effects in treated patients, and as selectable markers in gene therapy Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of the His6-tagged isozyme GST T1-1 in Escherichia coli Mus musculus
recombinant expression of the His6-tagged wild-type and mutant isozymes GST T1-1 in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
C14S/T226I/W234R construction of chimeric mutant F2:1215 comprising sequences from the human isozymes GST-T1-1 and GST-T2, as well as from the GST-T1-1 of Mus musculus, the mutant F2:1215 shows highly increased activity compared to the human enzyme and increased activity compared to the murine enzyme, overview Homo sapiens
additional information construction of chimeric mutants F2:1215 and F5:1474 comprising sequences from the murine isozymes GST-T1-1, as well as from the isozymes GST-T1-1 and GST-T2 of Homo sapiens, the mutant F2:1215 and mutant F5:1474 show increased activity compared to the human and to the murine enzyme, overview Mus musculus
W234R construction of chimeric mutant F5:1474 comprising sequences from the human isozymes GST-T1-1 and GST-T2, as well as from the GST-T1-1 of Mus musculus, the mutant F5:1474 shows increased activity compared to the human enzyme and to the murine enzyme, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics of recombinant His6-tagged isozyme GST T1-1, substrate 1,3-bis-(2-chloroethyl)-1-nitrosourea Mus musculus
additional information
-
 additional information steady-state kinetics of recombinant His6-tagged wild-type and mutant isozymes GST T1-1, substrate 1,3-bis-(2-chloroethyl)-1-nitrosourea Homo sapiens
0.6
-
 1,3-Bis-(2-chloroethyl)-1-nitrosourea pH 7.4, 37°C, recombinant mutant F2:1215 Mus musculus
0.6
-
 1,3-Bis-(2-chloroethyl)-1-nitrosourea pH 7.4, 37°C, recombinant mutant F2:1215 Homo sapiens
0.8
-
 1,3-Bis-(2-chloroethyl)-1-nitrosourea pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 Mus musculus
1
-
 1,3-Bis-(2-chloroethyl)-1-nitrosourea pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the human isozyme GST T1-1 is 80fold less active than the murine isozyme GST T1-1 ?
-
 ?
additional information Mus musculus the murine isozyme GST T1-1 is 80fold more active than the human isozyme GST T1-1 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isozyme GST T1-1
-
Mus musculus
-
 isozyme GST T1-1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione
-
 Mus musculus ?
-
 ?
1,2-epoxy-3-(4-nitrophenoxy)-propane + glutathione
-
 Homo sapiens ?
-
 ?
1,3-bis-(2-chloroethyl)-1-nitrosourea + glutathione
-
 Mus musculus ?
-
 ?
1,3-bis-(2-chloroethyl)-1-nitrosourea + glutathione
-
 Homo sapiens ?
-
 ?
4-nitrophenethyl bromide + glutathione
-
 Mus musculus ?
-
 ?
4-nitrophenethyl bromide + glutathione
-
 Homo sapiens ?
-
 ?
additional information the human isozyme GST T1-1 is 80fold less active than the murine isozyme GST T1-1 Homo sapiens ?
-
 ?
additional information the murine isozyme GST T1-1 is 80fold more active than the human isozyme GST T1-1 Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
GST T1-1
-
 Mus musculus
GST T1-1
-
 Homo sapiens
theta-class glutathione transferase
-
 Mus musculus
theta-class glutathione transferase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.035
-
 1,3-Bis-(2-chloroethyl)-1-nitrosourea pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 Homo sapiens
2.3
-
 1,3-Bis-(2-chloroethyl)-1-nitrosourea pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 Mus musculus
3.6
-
 1,3-Bis-(2-chloroethyl)-1-nitrosourea pH 7.4, 37°C, recombinant mutant F2:1215 Mus musculus
3.6
-
 1,3-Bis-(2-chloroethyl)-1-nitrosourea pH 7.4, 37°C, recombinant mutant F2:1215 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus
7.4
-
 assay at Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.036
-
 1,3-Bis-(2-chloroethyl)-1-nitrosourea pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 Homo sapiens
2.9
-
 1,3-Bis-(2-chloroethyl)-1-nitrosourea pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1 Mus musculus
6.3
-
 1,3-Bis-(2-chloroethyl)-1-nitrosourea pH 7.4, 37°C, recombinant mutant F2:1215 Mus musculus
6.3
-
 1,3-Bis-(2-chloroethyl)-1-nitrosourea pH 7.4, 37°C, recombinant mutant F2:1215 Homo sapiens