Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21 (DE3) | Laternula elliptica |
Crystallization (Comment) | Organism |
---|---|
enzyme in complex with substrates glutathione or 1-chloro-2,4-dinitrobenzene, sitting-drop vapour-diffusion method, 0.004 ml of protein solution, containing 20 mM Tris-HCl, pH 7.9, 200 mM NaCl, 2 mM DTT, is mixed with 0.004 ml of reservoir solution containing 6% Tacsimate, pH 8.0, and 26% w/v PEG 3350, for cocrystallization, the protein solution is mixed with glutathione at a molar ratio of 1:1 and with 1-chloro-2,4-dinitrobenzene at a molar ratio of 1:5. The glutathionecomplex crystals from 7% Tacsimate, pH 8.0, 24% w/v PEG 3350 and the CDNB-complex crystals from 8.5% Tacsimate, pH 8.0, 25% w/v PEG 335. Equilibration over 0.5 ml reservoir solution, 22°C, 3 days, X-ray diffraction structure determination and analysis at 2.2 A and 2.0 A resolution, respectively | Laternula elliptica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Laternula elliptica | - |
Antarctic soft-shelled clam | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration to over 95% purity | Laternula elliptica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Laternula elliptica | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | a rho-class glutathione S-transferase | Laternula elliptica |
GST | - |
Laternula elliptica |
rho-GST | - |
Laternula elliptica |