Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C14A/C47A/C101A | mutant shows 92% decrease in the specific activity towards 1-chloro-2,4-dinitrobenzene compared to the His-tagged wild type enzyme | Homo sapiens |
C14A/C47A/C101A/C169A | mutant shows 96% decrease in the specific activity towards 1-chloro-2,4-dinitrobenzene compared to the His-tagged wild type enzyme | Homo sapiens |
C47A/C101A | mutant shows 41% decrease in the specific activity towards 1-chloro-2,4-dinitrobenzene compared to the His-tagged wild type enzyme | Homo sapiens |
Y7F | mutant shows 99% decrease in the specific activity towards 1-chloro-2,4-dinitrobenzene compared to the His-tagged wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
glutathione | untagged recombinant wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens | |
0.37 | - |
glutathione | His-tagged recombinant wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens | |
0.53 | - |
glutathione | mutant enzyme C14A/C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens | |
0.66 | - |
1-chloro-2,4-dinitrobenzene | His-tagged recombinant wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens | |
0.88 | - |
1-chloro-2,4-dinitrobenzene | untagged recombinant wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens | |
0.97 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens | |
1.03 | - |
glutathione | mutant enzyme C14A/C47A/C101A/C169A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens | |
1.56 | - |
glutathione | mutant enzyme C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens | |
1.66 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme C14A/C47A/C101A/C169A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens | |
2.4 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme C14A/C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25500 | - |
His-tagged wild type enzyme, SDS-PAGE | Homo sapiens |
25630 | - |
His-tagged wild type enzyme, calculated from amino acid sequence | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09211 | - |
- |
Purification (Comment) | Organism |
---|---|
IDA-Ni2+ affinity column chromatography and glutathione Sepharose 4B column chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
mutant enzyme Y7F, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens |
2.52 | - |
mutant enzyme C14A/C47A/C101A/C169A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens |
4.75 | - |
mutant enzyme C14A/C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens |
36.13 | - |
mutant enzyme C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens |
61.07 | - |
His-tagged wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens |
71.7 | - |
untagged wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Homo sapiens | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase P1-1 | - |
Homo sapiens |
GSTP 1-1 | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme remains fairly stable up to 50°C and is inactivated irreversibly above 50°C | Homo sapiens |