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Literature summary for 2.5.1.18 extracted from

  • Wu, Y.; Shen, J.; Yin, Z.
    Expression, purification and functional analysis of hexahistidine-tagged human glutathione S-transferase P1-1 and its cysteinyl mutants (2007), Protein J., 26, 359-370.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C14A/C47A/C101A mutant shows 92% decrease in the specific activity towards 1-chloro-2,4-dinitrobenzene compared to the His-tagged wild type enzyme Homo sapiens
C14A/C47A/C101A/C169A mutant shows 96% decrease in the specific activity towards 1-chloro-2,4-dinitrobenzene compared to the His-tagged wild type enzyme Homo sapiens
C47A/C101A mutant shows 41% decrease in the specific activity towards 1-chloro-2,4-dinitrobenzene compared to the His-tagged wild type enzyme Homo sapiens
Y7F mutant shows 99% decrease in the specific activity towards 1-chloro-2,4-dinitrobenzene compared to the His-tagged wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
glutathione untagged recombinant wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens
0.37
-
glutathione His-tagged recombinant wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens
0.53
-
glutathione mutant enzyme C14A/C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens
0.66
-
1-chloro-2,4-dinitrobenzene His-tagged recombinant wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens
0.88
-
1-chloro-2,4-dinitrobenzene untagged recombinant wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens
0.97
-
1-chloro-2,4-dinitrobenzene mutant enzyme C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens
1.03
-
glutathione mutant enzyme C14A/C47A/C101A/C169A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens
1.56
-
glutathione mutant enzyme C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens
1.66
-
1-chloro-2,4-dinitrobenzene mutant enzyme C14A/C47A/C101A/C169A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens
2.4
-
1-chloro-2,4-dinitrobenzene mutant enzyme C14A/C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25500
-
His-tagged wild type enzyme, SDS-PAGE Homo sapiens
25630
-
His-tagged wild type enzyme, calculated from amino acid sequence Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P09211
-
-

Purification (Commentary)

Purification (Comment) Organism
IDA-Ni2+ affinity column chromatography and glutathione Sepharose 4B column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
mutant enzyme Y7F, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens
2.52
-
mutant enzyme C14A/C47A/C101A/C169A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens
4.75
-
mutant enzyme C14A/C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens
36.13
-
mutant enzyme C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens
61.07
-
His-tagged wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens
71.7
-
untagged wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
Homo sapiens S-(2,4-dinitrophenyl)glutathione + HCl
-
?

Synonyms

Synonyms Comment Organism
glutathione S-transferase P1-1
-
Homo sapiens
GSTP 1-1
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
the enzyme remains fairly stable up to 50°C and is inactivated irreversibly above 50°C Homo sapiens