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Literature summary for 2.5.1.18 extracted from
- MALDI mass sequencing and characterization of filarial glutathione-S-transferase (2007), Biochem. Biophys. Res. Commun., 356, 381-385.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alizarin | - |
Setaria cervi |  |
| alpha-tocopherol | significant inhibition | Setaria cervi | |
| bromosulfophthalein | - |
Setaria cervi | |
| Cibacron blue | significant inhibition | Setaria cervi | |
| ellagic acid | - |
Setaria cervi | |
| quercetin | - |
Setaria cervi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.61 | - |
glutathione | - |
Setaria cervi | |
| 2.22 | - |
1-chloro-2,4-dinitrobenzene | - |
Setaria cervi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25800 | - |
MALDI-TOF mass spectrometry | Setaria cervi |
| 26000 | - |
SDS-PAGE | Setaria cervi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Setaria cervi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| GSH-agarose affnity column chromatography, DEAE-Sephadex gel filtration, and GSH-agarose affinity column chromatography | Setaria cervi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
somatic extract | Setaria cervi |
| 1220 | - |
after 265.9fold purification | Setaria cervi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Setaria cervi | S-(2,4-dinitrophenyl)-glutathione + HCl | - |
? | |
| glutathione + cumene hydroperoxide | the enzyme has selenium-independent peroxidase activity | Setaria cervi | ? | - |
? | |
| glutathione + ethacrynic acid | - |
Setaria cervi | ? | - |
? | |
| additional information | no activity towards hydrogen peroxide, bromosulfophthalein, and 1,2-dichloro-4-nitrobenzene | Setaria cervi | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione-S-transferase | - |
Setaria cervi |
| GST-26 | - |
Setaria cervi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Setaria cervi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1515 | - |
1-chloro-2,4-dinitrobenzene | - |
Setaria cervi | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Setaria cervi |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
- |
Setaria cervi | Cibacron blue | |
| 0.25 | - |
- |
Setaria cervi | alpha-tocopherol | |
| 0.6 | - |
- |
Setaria cervi | ellagic acid | |
| 0.8 | - |
- |
Setaria cervi | bromosulfophthalein | |
| 5 | - |
- |
Setaria cervi | quercetin | |
| 12 | - |
- |
Setaria cervi | alizarin | |
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Release 2023.1 (January 2023)
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