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Literature summary for 2.5.1.18 extracted from
- A Mu-class glutathione S-transferase from gills of the marine shrimp Litopenaeus vannamei: purification and characterization (2007), J. Biochem. Mol. Toxicol., 21, 62-67.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | a sequential steady-state kinetic mechanism | Penaeus vannamei | |
| 0.335 | - |
glutathione | pH 6.5, 25°C | Penaeus vannamei |  |
| 0.39 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 25°C | Penaeus vannamei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
2 * 25000, SDS-PAGE | Penaeus vannamei |
| 50000 | - |
about, native PAGE | Penaeus vannamei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penaeus vannamei | - |
a marine shrimp, a mu class isozyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 106fold from gills by glutathione affinity chromatography to homogeneity | Penaeus vannamei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gill | - |
Penaeus vannamei | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 440.1 | - |
purified native enzyme | Penaeus vannamei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Penaeus vannamei | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 25000, SDS-PAGE | Penaeus vannamei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase | - |
Penaeus vannamei |
| GST | - |
Penaeus vannamei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.15 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 25°C | Penaeus vannamei | |
| 251.2 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 25°C | Penaeus vannamei | |
| 274.4 | - |
glutathione | pH 6.5, 25°C | Penaeus vannamei | |
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Release 2023.1 (January 2023)
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