Cloned (Comment) | Organism |
---|---|
gene bphK, expression in Escherichia coli strain GJ1158 | Paraburkholderia xenovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics using 1-chloro-2,4-dinitrobenzene as substrate | Paraburkholderia xenovorans | |
0.11 | - |
glutathione | pH 6.5, 25°C, recombinant enzyme, with (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | Paraburkholderia xenovorans | |
0.37 | - |
glutathione | pH 6.5, 25°C, recombinant enzyme, with (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | Paraburkholderia xenovorans | |
0.39 | - |
glutathione | pH 6.5, 25°C, recombinant enzyme, with (2Z,4E)-3-chloro-6-(3,5-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid | Paraburkholderia xenovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | Paraburkholderia xenovorans | - |
chloride + 5-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | - |
? | |
glutathione + (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | Paraburkholderia xenovorans | - |
chloride + 3-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | - |
? | |
glutathione + (2Z,4E)-3-chloro-6-(3,5-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid | Paraburkholderia xenovorans | - |
? | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | Paraburkholderia xenovorans | - |
chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
additional information | Paraburkholderia xenovorans | the enzyme catalyzes the dehalogenation of inhibitory metabolites of polychlorinated biphenyls and may be responsible for facilitation of PCB degradation by the bph pathway, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia xenovorans | - |
gene bphK | - |
Purification (Comment) | Organism |
---|---|
recombinant BphK from Escherichia coli strain GJ1158 by glutathione affinity chromatography | Paraburkholderia xenovorans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
RX + glutathione = HX + R-S-glutathione | the enzyme uses a rapid-equilibrium random-order ternary-complex mechanism or a compulsory order ternary-complex mechanism | Paraburkholderia xenovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | - |
Paraburkholderia xenovorans | chloride + 5-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | - |
? | |
glutathione + (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | - |
Paraburkholderia xenovorans | chloride + 3-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | - |
? | |
glutathione + (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | preferred polychlorinated biphenyl metabolite substrate | Paraburkholderia xenovorans | chloride + 3-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | - |
? | |
glutathione + (2Z,4E)-3-chloro-6-(3,5-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid | - |
Paraburkholderia xenovorans | ? | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Paraburkholderia xenovorans | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
additional information | the enzyme catalyzes the dehalogenation of inhibitory metabolites of polychlorinated biphenyls and may be responsible for facilitation of PCB degradation by the bph pathway, overview | Paraburkholderia xenovorans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BphK | - |
Paraburkholderia xenovorans |
glutathione S-transferase | - |
Paraburkholderia xenovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Paraburkholderia xenovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
glutathione | pH 6.5, 25°C, recombinant enzyme, with (2Z,4E)-3-chloro-6-(3,5-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid | Paraburkholderia xenovorans | |
0.16 | - |
glutathione | pH 6.5, 25°C, recombinant enzyme, with (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | Paraburkholderia xenovorans | |
0.24 | - |
glutathione | pH 6.5, 25°C, recombinant enzyme, with (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid | Paraburkholderia xenovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Paraburkholderia xenovorans |