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Literature summary for 2.5.1.18 extracted from

  • Hayeshi, R.; Mutingwende, I.; Mavengere, W.; Masiyanise, V.; Mukanganyama, S.
    The inhibition of human glutathione S-transferases activity by plant polyphenolic compounds ellagic acid and curcumin (2007), Food Chem. Toxicol., 45, 286-295.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of isozymes in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(+)-catechin hydrate inhibition of isozymes GST M1-1 and M2-2 Homo sapiens
curcumin inhibition of isozymes GST A1-1, A2-2, M1-1, M2-2, and P1-1 Homo sapiens
daidzein inhibition of isozymes GST A1-1, M1-1, and P1-1 Homo sapiens
ellagic acid inhibition of isozymes GST A1-1, A2-2, M1-1, M2-2, and P1-1, effects on substrate kinetics, overview Homo sapiens
ferulic acid inhibition of isozyme GST M1-1 Homo sapiens
genistein inhibition of isozymes GST M1-1 and M2-2 Homo sapiens
kaempferol inhibition of isozymes GST M1-1 and M2-2 Homo sapiens
quercetin inhibition of isozymes GST M1-1 and M2-2 Homo sapiens
resveratrol inhibition of isozymes GST A2-2, M1-1, M2-2, and P1-1 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.72
-
1-chloro-2,4-dinitrobenzene pH 7.4, 30°C, purified recombinant isozyme P1-1 Homo sapiens
0.86
-
glutathione pH 7.4, 30°C, purified recombinant isozyme P1-1 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
GST isozymes A1-1, A2-2, M1-1, M2-2, and P1-1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isozymes from Escherichia coli by S-hexylglutathione affinity chromatography to homogeneity Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
78
-
purified recombinant isozyme GST A2-2 Homo sapiens
84
-
purified recombinant isozyme GST A1-1 Homo sapiens
129
-
purified recombinant isozyme GST P1-1 Homo sapiens
157
-
purified recombinant isozyme GST M1-1 Homo sapiens
182
-
purified recombinant isozyme GST M2-2 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
Homo sapiens chloride + 2,4-dinitrophenyl-glutathione
-
?

Synonyms

Synonyms Comment Organism
glutathione S-transferase
-
Homo sapiens
GST
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
47.3
-
glutathione pH 7.4, 30°C, purified recombinant isozyme P1-1 Homo sapiens
82
-
1-chloro-2,4-dinitrobenzene pH 7.4, 30°C, purified recombinant isozyme P1-1 Homo sapiens
103.3
-
1-chloro-2,4-dinitrobenzene pH 7.4, 30°C, purified recombinant isozyme P1-1 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
-
Homo sapiens additional information
0.00004 0.005 pH 7.4, 30°C, purified recombinant isozymes GST A1-1, A2-2, M1-1, M2-2, and P1-1 Homo sapiens curcumin
0.00013 0.005 pH 7.4, 30°C, purified recombinant isozymes GST A1-1, A2-2, M1-1, M2-2, and P1-1 Homo sapiens ellagic acid
0.0003 0.0005 pH 7.4, 30°C, purified recombinant isozymes GST M1-1 and M2-2 Homo sapiens quercetin
0.001 0.002 pH 7.4, 30°C, purified recombinant isozymes GST M1-1 and M2-2 Homo sapiens kaempferol
0.004 0.005 pH 7.4, 30°C, purified recombinant isozymes GST M1-1 and M2-2 Homo sapiens genistein