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Literature summary for 2.5.1.18 extracted from

  • Na, B.K.; Kang, J.M.; Kim, T.S.; Sohn, W.M.
    Plasmodium vivax: molecular cloning, expression and characterization of glutathione S-transferase (2007), Exp. Parasitol., 116, 414-418.
    View publication on PubMed

Application

Application Comment Organism
drug development the enzyme is a target for drug development against the parasite Plasmodium vivax

Cloned(Commentary)

Cloned (Comment) Organism
gene gst, DNA and amino acid sequence determination and analysis, expression of wild-type enzyme in Escherichia coli strain DH5alpha, expression of His-tagged enzyme in Escherichia coli strain M15 Plasmodium vivax

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
glutathione pH 8.0, 37°C, recombinant enzyme Plasmodium vivax
2.1
-
1-chloro-2,4-dinitrobenzene pH 8.0, 37°C, recombinant enzyme Plasmodium vivax

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
2 * 25000, recombinant enzyme, SDS-PAGE Plasmodium vivax
50000
-
recombinant enzyme, gel filtration Plasmodium vivax

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Plasmodium vivax the enzyme is essential for parasite survival by protecting the parasite against oxidative stress and buffering the detoxification of heme-binding compounds ?
-
?

Organism

Organism UniProt Comment Textmining
Plasmodium vivax Q0ZS46 gene gst
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography, recombinant enzyme from Escherichia coli strain DH5alpha by gel filtration Plasmodium vivax

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.21
-
purified recombinant enzyme, substrate ethacrynic acid Plasmodium vivax
0.58
-
purified recombinant enzyme, substrate 1,2-dichloro-4-nitrobenzene Plasmodium vivax
34.7
-
purified recombinant enzyme, substrate 1-chloro-2,4-dinitrobenzene Plasmodium vivax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1,2-dichloro-4-nitrobenzene low activity compared to 1-chloro-2,4-dinitrobenzene Plasmodium vivax ?
-
?
glutathione + 1-chloro-2,4-dinitrobenzene preferred substrate Plasmodium vivax chloride + 2,4-dinitrophenyl-glutathione
-
?
glutathione + ethacrynic acid low activity compared to 1-chloro-2,4-dinitrobenzene Plasmodium vivax ?
-
?
additional information the enzyme is essential for parasite survival by protecting the parasite against oxidative stress and buffering the detoxification of heme-binding compounds Plasmodium vivax ?
-
?
additional information 2-nitrophenyl acetate, bromosulfophthalein, and cumene hydroperoxide are poor substrates, overview Plasmodium vivax ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 25000, recombinant enzyme, SDS-PAGE Plasmodium vivax

Synonyms

Synonyms Comment Organism
glutathione S-transferase
-
Plasmodium vivax
GST
-
Plasmodium vivax

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Plasmodium vivax

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
purified recombinant enzyme, quite stable Plasmodium vivax
37
-
purified recombinant enzyme, rapid decrease in activity Plasmodium vivax

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant enzyme Plasmodium vivax