Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbate | ascorbate enhances the inhibitory effects of SH reagents, overview, inactivation of total cytosolic GST activity from liver by the oxygen radical-generating system Cu2+/ascorbate, two mechanisms: ROS-induced oxidation and non-specific Cu2+ binding to protein thiol groups | Rattus norvegicus | |
Cu2+ | inhibitory in either the absence or presence of ascorbate, inactivation of total cytosolic GST activity from liver by the oxygen radical-generating system Cu2+/ascorbate, two mechanisms: ROS-induced oxidation and non-specific Cu2+ binding to protein thiol groups, the inhibition is prevented by glutathione but not 1-chloro-2,4-dinitrobenzene, Cu2+ inhibition affects the Km for glutathione but not of 1-chloro-2,4-dinitrobenzene, overview | Rattus norvegicus | |
H2O2 | inactivation of cytosolic GST activity from liver | Rattus norvegicus | |
Mersalyl acid | - |
Rattus norvegicus | |
NEM | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Cu2+ effects on Km, overview | Rattus norvegicus | |
0.11 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 25°C | Rattus norvegicus | |
0.19 | - |
glutathione | pH 6.5, 25°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Comment) | Organism |
---|---|
native enzyme partially by subcellular fractionation | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Rattus norvegicus | chloride + 2,4-dinitrophenyl-glutathione | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Rattus norvegicus |
GST | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
Mersalyl acid | pH 6.5, 25°C | Rattus norvegicus | |
0.2 | - |
NEM | pH 6.5, 25°C | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
- |
Rattus norvegicus | additional information | |
0.0051 | - |
pH 6.5, 25°C | Rattus norvegicus | Cu2+ |