Cloned (Comment) | Organism |
---|---|
gene gst, DNA and amino acid sequence determination and analysis, phylogenetic tree, sequence comparisons, overexpression of soluble enzyme in Escherichia coli | Hyphantria cunea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deltamethrin | an insecticide | Hyphantria cunea | |
fenitrothion | an insecticide | Hyphantria cunea | |
permethrin | an insecticide | Hyphantria cunea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Hyphantria cunea | |
0.41 | - |
1-chloro-2,4-dinitrobenzene | pH 8.0, 30°C, recombinant enzyme | Hyphantria cunea | |
0.53 | - |
4-hydroxynonenal | pH 8.0, 30°C, recombinant enzyme | Hyphantria cunea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23204 | - |
x * 23204, sequence calculation | Hyphantria cunea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyphantria cunea | Q4ACU7 | sigma class isozyme, gene gst; fall webworm, gene gst, sigma class isozyme | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli to homogeneity | Hyphantria cunea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | four-instar larvae | Hyphantria cunea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Hyphantria cunea | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
glutathione + 4-hydroxynonenal | - |
Hyphantria cunea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23204, sequence calculation | Hyphantria cunea |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Hyphantria cunea |
GST | - |
Hyphantria cunea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Hyphantria cunea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, purified recombinant enzyme, stable | Hyphantria cunea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Hyphantria cunea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | purified recombinant enzyme, 75% remaining activity | Hyphantria cunea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Hyphantria cunea | sequence calculation | - |
8.75 |