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Literature summary for 2.5.1.18 extracted from

  • O'Sullivan, S.M.; McCarthy, R.M.; Vargo, M.A.; Colman, R.F.; Sheehan, D.
    Chemical modification at subunit 1 of rat kidney alpha class glutathione transferase with 2,3,5,6-tetrachloro-1,4-benzoquinone: close structural connectivity between glutathione conjugation activity and non-substrate ligand binding (2006), Biochem. Pharmacol., 71, 1629-1636.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,3,5,6-tetrachloro-1,4-benzoquinone i.e. TCBQ, inhibition of GSTA1-2 and GSTA1-1 by 70-80%, and GSTA2-2 by 25%, binds to cysteine residues of the enzyme, e.g. Cys17 of GSTA1-1, via a first reversible step to covalent binding, overview, Kitz-Wilson inactivation model Rattus norvegicus
dinitrophenyl-glutathione
-
Rattus norvegicus
S-hexyl-glutathione
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol alpha class glutathione transferase Rattus norvegicus 5829
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
alpha class isozymes GSTA2-2, GSTA1-2 and GSTA1-1
-

Purification (Commentary)

Purification (Comment) Organism
native alpha class isozymes GSTA2-2, GSTA1-2 and GSTA1-1 from kidney by anion exchange and glutathione affinity chromatography, followed by another step of ion exchange chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney mainly alpha class glutathione transferase Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
Rattus norvegicus chloride + 2,4-dinitrophenyl-glutathione
-
?

Subunits

Subunits Comment Organism
More homology modeling of recombinant isozyme GSTA1-1 with and without bound inhibitor 2,3,5,6-tetrachloro-1,4-benzoquinone Rattus norvegicus

Synonyms

Synonyms Comment Organism
alpha class glutathione transferase
-
Rattus norvegicus
GST
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Rattus norvegicus