Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant N-terminally His6-tagged isozymes GST T1-1 in Escherichia coli strain XL-1 blue | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
W234K | site-directed mutagenesis, isozyme GST T1-1, the mutant shows altered substrate specificity compared to the wild-type enzyme | Homo sapiens |
W234R | site-directed mutagenesis, isozyme GST T1-1, amino acid exchange analogously to the murine enzyme, the mutant shows altered substrate specificity compared to the wild-type enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme GST T1-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant N-terminally His6-tagged isozymes GST T1-1 from Escherichia coli strain XL-1 blue by nickel affinity chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity of wild-type and mutant isozymes GST T1-1, overview | Homo sapiens |
4.96 | - |
purified wild-type GST T1-1, substrate 1-iodomethane | Homo sapiens |
7.84 | - |
purified wild-type GST T1-1, substrate 1,2-epoxy-3-(4-nitro-phenoxy)propane | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1,2-epoxy-3-(4-nitro-phenoxy)propane | best substrate for the wild-type GST T1-1 and mutant W234K | Homo sapiens | ? | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Homo sapiens | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
glutathione + 1-iodo-3-phenylpropane | - |
Homo sapiens | iodide + 3-phenylpropyl-glutathione | - |
? | |
glutathione + 1-iodobutane | - |
Homo sapiens | iodide + butyl-glutathione | - |
? | |
glutathione + 1-iodoethane | - |
Homo sapiens | iodide + ethylglutathione | - |
? | |
glutathione + 1-iodoheptane | - |
Homo sapiens | iodide + heptyl-glutathione | - |
? | |
glutathione + 1-iodohexane | - |
Homo sapiens | iodide + hexyl-glutathione | - |
? | |
glutathione + 1-iodomethane | best substrate of GST T1-1 mutant W234R | Homo sapiens | iodide + methylglutathione | - |
? | |
glutathione + 1-iodooctane | - |
Homo sapiens | iodide + octyl-glutathione | - |
? | |
glutathione + 1-iodopentane | - |
Homo sapiens | iodide + pentyl-glutathione | - |
? | |
glutathione + 1-iodopropane | - |
Homo sapiens | iodide + propyl-glutathione | - |
? | |
glutathione + 1-menaphthyl sulfate | - |
Homo sapiens | ? | - |
? | |
glutathione + 4-nitrobenzyl chloride | - |
Homo sapiens | chloride + 4-nitrobenzyl-glutathione | - |
? | |
glutathione + 4-nitrophenethyl bromide | - |
Homo sapiens | bromide + 4-nitrophenethyl-glutathione | - |
? | |
glutathione + cumene hydroperoxide | - |
Homo sapiens | ? | - |
? | |
glutathione + styrene 7,8-oxide | - |
Homo sapiens | ? | - |
? | |
additional information | substrate specificity of wild-type and mutant isozymes GST T1-1, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GST | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |