Protein Variants | Comment | Organism |
---|---|---|
Y108A | mutant enzyme shows 58% of the wild-type activity with 1,2-dichloro-4-nitrobenzene as substrate and 37% of the wild-type activity with 1,2-epoxy-3-(p-nitrophenoxy)propane, 2% of the wild-type GSH peroxidase activity towards cumene hydroperoxide and 163% of the wild-type steroid isomerase activity towards DELTA5-androstene-3,17-dione | Homo sapiens |
Y108F | mutant enzyme is as active as wild-type enzyme with 1,2-dichloro-4-nitrobenzene as substrate and shows 7% of the wild-type activity with 1,2-epoxy-3-(p-nitrophenoxy)propane, 77% of the wild-type GSH peroxidase activity towards cumene hydroperoxide and 149% of the wild-type steroid isomerase activity towards DELTA5-androstene-3,17-dione. Mutation results in an approximately 3fold decrease in the I50 value of S-methyl-GSH | Homo sapiens |
Y108W | mutant enzyme shows 215% of the wild-type activity with 1,2-dichloro-4-nitrobenzene as substrate and 35% of the wild-type activity with 1,2-epoxy-3-(p-nitrophenoxy)propane, 16% of the wild-type GSH peroxidase activity towards cumene hydroperoxide and 36% of the wild-type steroid isomerase activity towards DELTA5-androstene-3,17-dione. Mutation results in an approximately 3fold decrease in the I50 value of S-methyl-GSH. Lower heat stability than wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hematin | - |
Homo sapiens | |
S-(2,4-dinitrophenyl)GSH | - |
Homo sapiens | |
S-methyl-GSH | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
GSH | 30°C, mutant enzyme Y108F, reaction with 1,2-dichloro-4-nitrobenzene | Homo sapiens | |
0.09 | - |
GSH | 30°C, mutant enzyme Y108W, reaction with 1,2-dichloro-4-nitrobenzene | Homo sapiens | |
0.13 | - |
1,2-epoxy-3-(p-nitrophenoxy)propane | 30°C, mutant enzyme Y108W | Homo sapiens | |
0.16 | - |
GSH | 30°C, mutant enzyme Y108F, reaction with cumene hydroperoxide | Homo sapiens | |
0.17 | - |
GSH | 30°C, wild-type enzyme, reaction with cumene hydroperoxide | Homo sapiens | |
0.2 | - |
GSH | 30°C, mutant enzyme Y108W, reaction with cumene hydroperoxide | Homo sapiens | |
0.25 | - |
1,2-epoxy-3-(p-nitrophenoxy)propane | 30°C, wild-type enzyme | Homo sapiens | |
0.27 | - |
1,2-dichloro-4-nitrobenzene | 30°C, mutant enzyme Y108W | Homo sapiens | |
0.29 | - |
GSH | 30°C, wild-type enzyme, reaction with 1,2-dichloro-4-nitrobenzene | Homo sapiens | |
0.3 | - |
cumene hydroperoxide | 30°C, wild-type enzyme | Homo sapiens | |
0.4 | - |
GSH | 30°C, mutant enzyme Y108A, reaction with 1,2-dichloro-4-nitrobenzene | Homo sapiens | |
0.4 | - |
GSH | 30°C, mutant enzyme Y108A, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane | Homo sapiens | |
0.4 | - |
GSH | 30°C, wild-type enzyme, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane | Homo sapiens | |
0.45 | - |
1,2-dichloro-4-nitrobenzene | 30°C, mutant enzyme Y108F | Homo sapiens | |
0.46 | - |
GSH | 30°C, mutant enzyme Y108F, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane | Homo sapiens | |
0.48 | - |
GSH | 30°C, mutant enzyme Y108W, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane | Homo sapiens | |
0.65 | - |
1,2-dichloro-4-nitrobenzene | 30°C, wild-type enzyme | Homo sapiens | |
0.84 | - |
1,2-dichloro-4-nitrobenzene | 30°C, mutant enzyme Y108A | Homo sapiens | |
0.88 | - |
GSH | 30°C, pH 6.5, wild-type enzyme, reaction with DELTA5-androstene-3,17-dione | Homo sapiens | |
0.9 | - |
cumene hydroperoxide | 30°C, mutant enzyme Y108W | Homo sapiens | |
1.3 | - |
1,2-epoxy-3-(p-nitrophenoxy)propane | 30°C, mutant enzyme Y108A | Homo sapiens | |
1.76 | - |
GSH | 30°C, mutant enzyme Y108A, reaction with cumene hydroperoxide | Homo sapiens | |
1.84 | - |
1,2-epoxy-3-(p-nitrophenoxy)propane | 30°C, mutant enzyme Y108F | Homo sapiens | |
2.11 | - |
cumene hydroperoxide | 30°C, mutant enzyme Y108F | Homo sapiens | |
8 | - |
cumene hydroperoxide | 30°C, mutant enzyme Y108A | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
enzyme form glutathione S-transferase P1-1, recombinant | - |
Purification (Comment) | Organism |
---|---|
recombinant | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dichloro-4-nitrobenzene + GSH | - |
Homo sapiens | ? | - |
? | |
1,2-epoxy-3-(p-nitrophenoxy)propane + GSH | - |
Homo sapiens | ? | - |
? | |
cumene hydroperoxide + GSH | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase P1-1 | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7, 10 min, wild-type enzyme and mutant enzymes Y108A and Y108F are stable up to, inactivation above | Homo sapiens |
53 | - |
midpoint of inactivation of mutant enzyme Y108W | Homo sapiens |
77 | - |
midpoint of inactivation of wild-type enzyme | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31 | - |
1,2-epoxy-3-(p-nitrophenoxy)propane | 30°C, mutant enzyme Y108F | Homo sapiens | |
107 | - |
GSH | 30°C, mutant enzyme Y108F, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane | Homo sapiens | |
108 | - |
GSH | 30°C, wild-type enzyme, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane | Homo sapiens | |
110 | - |
1,2-dichloro-4-nitrobenzene | 30°C, mutant enzyme Y108A | Homo sapiens | |
110 | - |
1,2-dichloro-4-nitrobenzene | 30°C, mutant enzyme Y108F | Homo sapiens | |
110 | - |
GSH | 30°C, mutant enzyme Y108F, reaction with 1,2-dichloro-4-nitrobenzene | Homo sapiens | |
112 | - |
1,2-epoxy-3-(p-nitrophenoxy)propane | 30°C, mutant enzyme Y108A | Homo sapiens | |
132 | - |
GSH | 30°C, mutant enzyme Y108A, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane | Homo sapiens | |
150 | - |
GSH | 30°C, mutant enzyme Y108W, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane | Homo sapiens | |
200 | - |
cumene hydroperoxide | 30°C, mutant enzyme Y108A | Homo sapiens | |
201 | - |
1,2-epoxy-3-(p-nitrophenoxy)propane | 30°C, wild-type enzyme | Homo sapiens | |
210 | - |
1,2-dichloro-4-nitrobenzene | 30°C, wild-type enzyme | Homo sapiens | |
302 | - |
1,2-epoxy-3-(p-nitrophenoxy)propane | 30°C, mutant enzyme Y108W | Homo sapiens | |
320 | - |
1,2-dichloro-4-nitrobenzene | 30°C, mutant enzyme Y108W | Homo sapiens | |
410 | - |
GSH | 30°C, mutant enzyme Y108A, reaction with 1,2-dichloro-4-nitrobenzene | Homo sapiens | |
410 | - |
GSH | 30°C, wild-type enzyme, reaction with 1,2-dichloro-4-nitrobenzene | Homo sapiens | |
700 | - |
GSH | 30°C, mutant enzyme Y108W, reaction with 1,2-dichloro-4-nitrobenzene | Homo sapiens | |
1020 | - |
GSH | 30°C, mutant enzyme Y108F, reaction with cumene hydroperoxide | Homo sapiens | |
1370 | - |
GSH | 30°C, wild-type enzyme, reaction with cumene hydroperoxide | Homo sapiens | |
1610 | - |
GSH | 30°C, mutant enzyme Y108W, reaction with cumene hydroperoxide | Homo sapiens | |
1700 | - |
GSH | 30°C, mutant enzyme Y108A, reaction with cumene hydroperoxide | Homo sapiens | |
2700 | - |
cumene hydroperoxide | 30°C, mutant enzyme Y108W | Homo sapiens | |
8100 | - |
cumene hydroperoxide | 30°C, mutant enzyme Y108F | Homo sapiens | |
9600 | - |
cumene hydroperoxide | 30°C, wild-type enzyme | Homo sapiens |