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Literature summary for 2.5.1.18 extracted from

  • Vararattanavech, A.; Prommeenate, P.; Ketterman, A.J.
    The structural roles of a conserved small hydrophobic core in the active site and an ionic bridge in domain I of Delta class glutathione S-transferase (2006), Biochem. J., 393, 89-95.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Anopheles dirus

Protein Variants

Protein Variants Comment Organism
E37A half-life at 45°C is 1.73 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.3fold lower than wild-type value Anopheles dirus
E37Q half-life at 45°C is 6.54 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is nearly identical to wild-type value Anopheles dirus
I52A half-life at 45°C is 9.58 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 100fold lower than wild-type value Anopheles dirus
I52L half-life at 45°C is 18.1 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.4fold lower than wild-type value Anopheles dirus
L33A half-life at 45°C is 45.3 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 2.7fold lower than wild-type value Anopheles dirus
L33F half-life at 45°C is 212 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 40.2fold lower than wild-type value Anopheles dirus
L33I half-life at 45°C is 30.4 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.2fold lower than wild-type value Anopheles dirus
L33Y half-life at 45°C is 71.7 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 186fold lower than wild-type value Anopheles dirus
L6A half-life at 45°C is 1.73 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.5fold lower than wild-type value Anopheles dirus
T31A half-life at 45°C is 22.7 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 2.7fold lower than wild-type value Anopheles dirus

General Stability

General Stability Organism
wild-type enzyme becomes completely unfolded after incubation for 10 min in 4 M guanidinium chloride Anopheles dirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
reduced glutathione mutant enzyme I52L Anopheles dirus
0.31
-
reduced glutathione mutant enzyme L6A Anopheles dirus
0.32
-
1-chloro-2,4-dinitrobenzene mutant enzyme I52A Anopheles dirus
0.42
-
reduced glutathione mutant enzyme L33I Anopheles dirus
0.5
-
1-chloro-2,4-dinitrobenzene wild-type enzyme Anopheles dirus
0.5
-
reduced glutathione wild-type enzyme Anopheles dirus
0.52
-
1-chloro-2,4-dinitrobenzene mutant enzyme A35R Anopheles dirus
0.54
-
1-chloro-2,4-dinitrobenzene mutant enzyme E37A Anopheles dirus
0.57
-
1-chloro-2,4-dinitrobenzene mutant enzyme K42A Anopheles dirus
0.58
-
1-chloro-2,4-dinitrobenzene mutant enzyme E37Q Anopheles dirus
0.62
-
1-chloro-2,4-dinitrobenzene mutant enzyme K40A Anopheles dirus
0.63
-
reduced glutathione mutant enzyme K42A Anopheles dirus
0.73
-
reduced glutathione mutant enzyme A35R Anopheles dirus
0.73
-
1-chloro-2,4-dinitrobenzene mutant enzyme L33I Anopheles dirus
0.74
-
1-chloro-2,4-dinitrobenzene mutant enzyme I52L Anopheles dirus
0.77
-
1-chloro-2,4-dinitrobenzene mutant enzyme T31A Anopheles dirus
0.81
-
1-chloro-2,4-dinitrobenzene mutant enzyme L33Y Anopheles dirus
1.1
-
reduced glutathione mutant enzyme L33Y Anopheles dirus
1.14
-
reduced glutathione mutant enzyme K40A Anopheles dirus
1.17
-
1-chloro-2,4-dinitrobenzene mutant enzyme L33A Anopheles dirus
1.3
-
1-chloro-2,4-dinitrobenzene mutant enzyme L33F Anopheles dirus
1.49
-
1-chloro-2,4-dinitrobenzene mutant enzyme L6A Anopheles dirus
1.85
-
reduced glutathione mutant enzyme E37A Anopheles dirus
2.26
-
reduced glutathione mutant enzyme E37Q Anopheles dirus
2.42
-
reduced glutathione mutant enzyme L33F Anopheles dirus
7.04
-
reduced glutathione mutant enzyme I52A Anopheles dirus
8.2
-
reduced glutathione mutant enzyme L33A Anopheles dirus
11.4
-
reduced glutathione mutant enzyme T31A Anopheles dirus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
x * 25000, SDS-PAGE Anopheles dirus

Organism

Organism UniProt Comment Textmining
Anopheles dirus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Anopheles dirus

Renatured (Commentary)

Renatured (Comment) Organism
wild-type enzyme becomes completely unfolded after incubation for 10 min in 4 M guanidinium chloride. Refolding rate of wild-type enzyme is 0.54 per min, 20.7% recovery Anopheles dirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced glutathione + 1,2-dichloro-4-nitrobenzene
-
Anopheles dirus ?
-
?
reduced glutathione + 1-chloro-2,4-dinitrobenzene
-
Anopheles dirus S-2,4-dinitrophenylglutathione + HCl
-
?
reduced glutathione + 4-nitrobenzyl chloride
-
Anopheles dirus ?
-
?
reduced glutathione + 4-nitrophenethyl bromide
-
Anopheles dirus ?
-
?
reduced glutathione + ethacrynic acid
-
Anopheles dirus ?
-
?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Anopheles dirus

Synonyms

Synonyms Comment Organism
adGSTD4-4
-
Anopheles dirus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
half-life: 15.3 min (wild-type enzyme), 45.3 min (mutant enzyme L33A), 71.7 min (mutant enzyme L33Y), 212 min (mutant enzyme L33F), 30.4 min (mutant enzyme L33I), 1.73 min (mutant enzyme L6A), 22.7 min (mutant enzyme T31A), 9.58 min (mutant enzyme I52A), 18.1 min (mutant enzyme I52L), 1.73 min (mutant enzyme L6A), 6.54 min (mutant enzyme E37Q), 3.94 min (mutant enzyme K40A), 8.44 min (mutant enzyme E42A), 9.01 min (mutant enzyme A35R) Anopheles dirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
1-chloro-2,4-dinitrobenzene mutant enzyme L33Y Anopheles dirus
0.14
-
reduced glutathione mutant enzyme L33Y Anopheles dirus
0.26
-
1-chloro-2,4-dinitrobenzene mutant enzyme I52A Anopheles dirus
0.26
-
reduced glutathione mutant enzyme I52A Anopheles dirus
0.65
-
1-chloro-2,4-dinitrobenzene mutant enzyme L33F Anopheles dirus
0.65
-
reduced glutathione mutant enzyme L33F Anopheles dirus
9.7
-
1-chloro-2,4-dinitrobenzene mutant enzyme L33A Anopheles dirus
9.7
-
reduced glutathione mutant enzyme L33A Anopheles dirus
9.73
-
1-chloro-2,4-dinitrobenzene mutant enzyme T31A Anopheles dirus
9.73
-
reduced glutathione mutant enzyme T31A Anopheles dirus
17.7
-
1-chloro-2,4-dinitrobenzene mutant enzyme L6A Anopheles dirus
17.7
-
reduced glutathione mutant enzyme L6A Anopheles dirus
18.3
-
1-chloro-2,4-dinitrobenzene mutant enzyme I52L Anopheles dirus
18.3
-
reduced glutathione mutant enzyme I52L Anopheles dirus
20.4
-
1-chloro-2,4-dinitrobenzene mutant enzyme E37A Anopheles dirus
20.4
-
reduced glutathione mutant enzyme E37A Anopheles dirus
21.5
-
1-chloro-2,4-dinitrobenzene mutant enzyme K40A Anopheles dirus
21.5
-
reduced glutathione mutant enzyme K40A Anopheles dirus
22.2
-
1-chloro-2,4-dinitrobenzene mutant enzyme L33I Anopheles dirus
22.2
-
reduced glutathione mutant enzyme L33I Anopheles dirus
22.4
-
1-chloro-2,4-dinitrobenzene mutant enzyme A35R Anopheles dirus
22.4
-
reduced glutathione mutant enzyme A35R Anopheles dirus
26.1
-
1-chloro-2,4-dinitrobenzene wild-type enzyme Anopheles dirus
26.1
-
reduced glutathione wild-type enzyme Anopheles dirus
26.3
-
1-chloro-2,4-dinitrobenzene mutant enzyme E37Q Anopheles dirus
26.3
-
reduced glutathione mutant enzyme E37Q Anopheles dirus
26.5
-
1-chloro-2,4-dinitrobenzene mutant enzyme K42A Anopheles dirus
26.5
-
reduced glutathione mutant enzyme K42A Anopheles dirus