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Literature summary for 2.5.1.18 extracted from

  • Wongsantichon, J.; Harnnoi, T.; Ketterman, A.J.
    A sensitive core region in the structure of glutathione S-transferases (2003), Biochem. J., 373, 759-765.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes Anopheles dirus

Protein Variants

Protein Variants Comment Organism
L103E the ratio of turnover number to KM-value for 1-chloro-2,4-dinitrobenzene is 1.8fold higher than wild-type ratio, the ratio of turnover number to KM-value for GSH is 2.9fold lower than wild-type ratio. Half-life of mutant enzyme at 45°C is below 1 min, compared to half-life of wild-type enzyme, which is about 13 min Anopheles dirus
L103I the ratio of turnover number to KM-value for 1-chloro-2,4-dinitrobenzene is 3fold higher than wild-type ratio, the ratio of turnover number to KM-value for GSH is 2.7fold lower than wild-type ratio. Half-life of mutant enzyme at 45°C is below 1 min, compared to half-life of wild-type enzyme, which is about 13 min Anopheles dirus
L103M the ratio of turnover number to KM-value for 1-chloro-2,4-dinitrobenzene is 3.6fold higher than wild-type ratio, the ratio of turnover number to KM-value for GSH is 5.7fold lower than wild-type ratio. Increased half-life at 45°C as compared to wild-type enzyme Anopheles dirus
L103N expressed as insoluble inclusion bodies in Escherichia coli. The ratio of turnover number to KM-value for 1-chloro-2,4-dinitrobenzene is 1.15fold higher than wild-type ratio, the ratio of turnover number to KM-value for GSH is 44.3fold lower than wild-type ratio. Half-life of mutant enzyme at 45°C is below 1 min, compared to half-life of wild-type enzyme, which is about 13 min Anopheles dirus
L103R the ratio of turnover number to KM-value for 1-chloro-2,4-dinitrobenzene is fold higher than wild-type ratio, the ratio of turnover number to KM-value for GSH is fold lower than wild-type ratio. Half-life of mutant enzyme at 45°C is below 1 min, compared to half-life of wild-type enzyme, which is about 13 min Anopheles dirus
L103Y expressed as insoluble inclusion bodies in Escherichia coli. The ratio of turnover number to KM-value for 1-chloro-2,4-dinitrobenzene is fold higher than wild-type ratio, the ratio of turnover number to KM-value for GSH is fold lower than wild-type ratio. Half-life of mutant enzyme at 45°C is below 1 min, compared to half-life of wild-type enzyme, which is about 13 min Anopheles dirus
L104A the ratio of turnover number to KM-value for 1-chloro-2,4-dinitrobenzene is 1.3fold higher than wild-type ratio, the ratio of turnover number to KM-value for GSH is 33.3fold lower than wild-type ratio. Half-life of mutant enzyme at 45°C is below 1 min, compared to half-life of wild-type enzyme, which is about 13 min Anopheles dirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.49
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103I Anopheles dirus
0.62
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103A Anopheles dirus
0.7
-
 GSH wild-type enzyme Anopheles dirus
0.72
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103E Anopheles dirus
0.76
-
 1-chloro-2,4-dinitrobenzene wild-type enzyme Anopheles dirus
0.77
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103N Anopheles dirus
0.91
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103M Anopheles dirus
3.42
-
 GSH mutant enzyme L103E Anopheles dirus
3.7
-
 GSH mutant enzyme L103I Anopheles dirus
6.38
-
 GSH mutant enzyme L103M Anopheles dirus
24
-
 GSH mutant enzyme L103A Anopheles dirus
37.4
-
 GSH mutant enzyme L103N Anopheles dirus

Organism

Organism UniProt Comment Textmining
Anopheles dirus
-
 enzyme form AdGSTD4-4
-

Purification (Commentary)

Purification (Comment) Organism
-
 Anopheles dirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione
-
 Anopheles dirus S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?

Synonyms

Synonyms Comment Organism
adGSTD4-4
-
 Anopheles dirus
glutathione S-transferase
-
 Anopheles dirus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 half-life of wild-type enzyme is about 13 min, half-life of mutant enzyme L103M is about 15 min, half-life of mutant enzymes L103N, L103A, L103E and l103I is below 1 min Anopheles dirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.6
-
 GSH wild-type enzyme Anopheles dirus
18.6
-
 1-chloro-2,4-dinitrobenzene wild-type enzyme Anopheles dirus
19.78
-
 GSH mutant enzyme L103A Anopheles dirus
19.78
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103A Anopheles dirus
21.94
-
 GSH mutant enzyme L103N Anopheles dirus
21.94
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103N Anopheles dirus
30.16
-
 GSH mutant enzyme L103M Anopheles dirus
30.16
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103M Anopheles dirus
31.9
-
 GSH mutant enzyme L103E Anopheles dirus
31.9
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103E Anopheles dirus
36.21
-
 GSH mutant enzyme L103I Anopheles dirus
36.21
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103I Anopheles dirus