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Literature summary for 2.5.1.18 extracted from

  • Flury, T.; Daam, D.; Kreuz, K.
    A 2,4-D-inducible glutathione S-transferase from soybean (Glycine max). Purification, characterization and induction (1995), Physiol. Plant., 94, 312-318.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
metolachlor
-
Glycine max
0.232
-
glutathione
-
Glycine max
0.503
-
1-chloro-2,4-dinitrobenzene
-
Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
2 * 26000, SDS-PAGE Glycine max
49000
-
gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
near homogeneity, 2,4-dichloro-phenoxyacetic acid-inducible GST isoenzyme Glycine max

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.024
-
with metolachlor as substrate Glycine max
4.9
-
with 1,2-dichloro-4-nitrobenzene as substrate Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
Glycine max S-2,4-dinitrophenylglutathione + HCl
-
?
glutathione + metolachlor herbicide Glycine max ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 26000, SDS-PAGE Glycine max