Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hemin | competitive with 1-chloro-2,4-dinitrobenzene, non-competitive with glutathione | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
reduced glutathione | - |
Gallus gallus | |
1.92 | - |
1-chloro-2,4-dinitrobenzene | - |
Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Gallus gallus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
2 * 23000, SDS-PAGE | Gallus gallus |
45000 | - |
gel filtration | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | foetal | Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.03 | - |
with 1-chloro-2,4-dinitrobenzene + glutathione or trans-4-phenyl-3-buten-2-one as substrate | Gallus gallus |
0.49 | - |
with 1,2-epoxy-3-(4-nitrophenoxy)propane as substrate | Gallus gallus |
0.75 | - |
with 4-nitrobenzyl chloride as substrate | Gallus gallus |
0.83 | - |
with ethacrynic acid as substrate | Gallus gallus |
3.44 | - |
- |
Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1,2-dichloro-4-nitrobenzene | - |
Gallus gallus | ? | - |
? | |
glutathione + 1,2-epoxy-3-(4-nitrophenoxy)propane | - |
Gallus gallus | ? | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Gallus gallus | S-2,4-dinitrophenylglutathione + HCl | - |
? | |
glutathione + 4-nitrobenzyl chloride | - |
Gallus gallus | ? | - |
? | |
glutathione + ethacrynic acid | - |
Gallus gallus | ? | - |
? | |
glutathione + trans-4-phenyl-3-buten-2-one | - |
Gallus gallus | ? | - |
? | |
additional information | obligate requirement for reduced glutathione as thiol donor | Gallus gallus | ? | - |
? | |
RX + glutathione | RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange | Gallus gallus | HX + R-S-glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 23000, SDS-PAGE | Gallus gallus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, 50% loss of activity | Gallus gallus |
80 | - |
complete inactivation after 10 min | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Gallus gallus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
hemin | with reduced glutathione | Gallus gallus |