Application | Comment | Organism |
---|---|---|
medicine | DHPS is an antimicrobial therapeutic target by chemical and genetic means | Burkholderia cenocepacia |
Cloned (Comment) | Organism |
---|---|
recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Burkholderia cenocepacia |
Crystallization (Comment) | Organism |
---|---|
purified recombinant apo-enzyme and enzyme in complex with inhibitor sulphadoxine or product 7,8-dihydropteroate, sitting drop vapor diffusion method, mixing of 0.001 ml of protein solution, containing 7.5 mg/ml protein in 50 mM Tris-HCl, 250 mM NaCl pH 7.5, with 0.001 ml of reservoir solution, containing 0.1 M Tris-HCl, pH 8, 10% PEG 8000, 0.3 M MgCl2, 4°C, X-ray diffraction structure determinationation and analysis at 1.95-2.35 A resolution, molecular replacement and modeling | Burkholderia cenocepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
recombinant enzyme, gel filtration | Burkholderia cenocepacia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate | Burkholderia cenocepacia | - |
diphosphate + 7,8-dihydropteroate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cenocepacia | A0A0H2XP43 | - |
- |
no activity in Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)by nickel affinity chromatography and gel filtration | Burkholderia cenocepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate | - |
Burkholderia cenocepacia | diphosphate + 7,8-dihydropteroate | - |
r | |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate | - |
Burkholderia cenocepacia | diphosphate + 7,8-dihydropteroate | 7,8-dihydropteroate binding mode, overview | r |
Subunits | Comment | Organism |
---|---|---|
dimer | structure and sequence comparison, overview | Burkholderia cenocepacia |
Synonyms | Comment | Organism |
---|---|---|
DHPS | - |
Burkholderia cenocepacia |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme supports the biosynthesis of folate, a key metabolite required to support the synthesis of DNA, and proteins | Burkholderia cenocepacia |
additional information | analysis of DHPS active site and interactions with the enzyme product 7,8-dihydropteroate, overview. DHPS shows plasticity near the substrate-binding pocket and a range of loop conformations that contribute to the architecture of the DHPS active site | Burkholderia cenocepacia |