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Literature summary for 2.5.1.15 extracted from
- Examination of intrinsic sulfonamide resistance in Bacillus anthracis: a novel assay for dihydropteroate synthase (2008), Biochim. Biophys. Acta, 1780, 848-853.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Sulfadiazine | reaction rate increased by sulfadiazine sodium salt, sulfamethoxazole, sulfamethazole, sulfanilamide, sulfathiazole sodium salt in presence of both substrates | Bacillus anthracis |  |
| sulfamethoxazole | reaction rate increased by sulfadiazine sodium salt, sulfamethoxazole, sulfamethazole, sulfanilamide, sulfathiazole sodium salt in presence of both substrates | Bacillus anthracis | |
| sulfanilamide | reaction rate increased by sulfadiazine sodium salt, sulfamethoxazole, sulfamethazole, sulfanilamide, sulfathiazole sodium salt in presence of both substrates | Bacillus anthracis | |
| sulfathiazole | reaction rate increased by sulfadiazine sodium salt, sulfamethoxazole, sulfamethazole, sulfanilamide, sulfathiazole sodium salt in presence of both substrates | Bacillus anthracis | |
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | established coupled enzymatic assay for kinetic analyses of DHPS activity (coupled to pyrophosphate-dependent phosphofructokinase, aldolase, triosephosphate isomerase, alpha-glycerophosphate dehydrogenase) in presence or absence of activity-modulating compounds | Bacillus anthracis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-hydroxymethylpterin monophosphate | 6HMP, competitive inhibitor, 6-hydroxymethyl-7,8-dihydropterin diphosphate analogue | Bacillus anthracis | |
| additional information | no inhibition by sulfadiazine sodium salt, sulfamethoxazole, sulfamethazole, sulfanilamide, sulfathiazole sodium salt in presence of both substrates | Bacillus anthracis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000058 | - |
dapson | in absence of 4-aminobenzoate | Bacillus anthracis | |
| 0.00033 | - |
sulfathiazole | in absence of 4-aminobenzoate | Bacillus anthracis | |
| 0.0006 | - |
sulfanilamide | in absence of 4-aminobenzoate | Bacillus anthracis | |
| 0.0007 | - |
sulfamethizole | in absence of 4-aminobenzoate | Bacillus anthracis | |
| 0.0015 | - |
sulfisoxazole | in absence of 4-aminobenzoate | Bacillus anthracis | |
| 0.0026 | - |
4-Aminobenzoate | 20 microM DHP-PP, 25 °C | Bacillus anthracis | |
| 0.0027 | - |
6-hydroxymethyl-7,8-dihydropterin diphosphate | 100 microM 4-aminobenzoate, 25 °C | Bacillus anthracis | |
| 0.0027 | - |
sulfamethoxazole | in absence of 4-aminobenzoate | Bacillus anthracis | |
| 0.00289 | - |
Sulfadiazine | in absence of 4-aminobenzoate | Bacillus anthracis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus anthracis | Q81VW8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-tagged recombinant protein by fast-performance liquid chromatography | Bacillus anthracis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0.906 +/-0.002 microM/min, with respect to dapson, in absence of 4-aminobenzoate | Bacillus anthracis |
| additional information | - |
1.24 +/-0.03 microM/min, with respect to sulfamethizole, in absence of 4-aminobenzoate | Bacillus anthracis |
| additional information | - |
1.26 +/-0.07 microM/min, with respect to sulfanilamide, in absence of 4-aminobenzoate | Bacillus anthracis |
| additional information | - |
1.39 +/-0.01 microM/min, with respect to sulfathiazole, in absence of 4-aminobenzoate | Bacillus anthracis |
| additional information | - |
1.71 +/-0.03 microM/min, with respect to 4-aminobenzoate | Bacillus anthracis |
| additional information | - |
1.80 +/-0.03 microM/min, with respect to sulfisoxazole, in absence of 4-aminobenzoate | Bacillus anthracis |
| additional information | - |
1.88 +/-0.05 microM/min, with respect to sulfamethoxazole, in absence of 4-aminobenzoate | Bacillus anthracis |
| additional information | - |
1.947 +/-0.03 microM/min, with respect to sulfadiazine, in absence of 4-aminobenzoate | Bacillus anthracis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate | pH 7.5, folate biosynthesis | Bacillus anthracis | diphosphate + 7,8-dihydropteroate | coupled with diphosphate-dependent phosphofructokinase, aldolase, triosephosphate isomerase, alpha-glycerophosphate dehydrogenase, in presence of NADH and D-fructose 6-phosphate, decrease in NADH monitored as change of absorbance at 340 nm | ? | |
| dapson + Mg-6-hydroxymethyl-7,8-dihydropterin diphosphate | - |
Bacillus anthracis | diphosphate + 2-amino-6-[([4-[(4-aminophenyl)sulfonyl]phenyl]amino)methyl]-7,8-dihydropteridin-4(3H)-one | - |
? | |
| sulfadiazine + 6-hydroxymethyl-7,8-dihydropterin diphosphate | - |
Bacillus anthracis | diphosphate + 4-[[(2-amino-4-oxo-3,4,7,8-tetrahydropteridin-6-yl)methyl]amino]-N-pyrimidin-2-ylbenzenesulfonamide | - |
? | |
| sulfamethizole + 6-hydroxymethyl-7,8-dihydropterin diphosphate | - |
Bacillus anthracis | diphosphate + 4-[[(2-amino-4-oxo-3,4,7,8-tetrahydropteridin-6-yl)methyl]amino]-N-(5-methyl-1,3,4-thiadiazol-2-yl)benzenesulfonamide | - |
? | |
| sulfamethizole + 6-hydroxymethyl-7,8-dihydropterin diphosphate | - |
Bacillus anthracis | ? | - |
? | |
| sulfamethoxazole + 6-hydroxymethyl-7,8-dihydropterin diphosphate | - |
Bacillus anthracis | diphosphate + 4-[[(2-amino-4-oxo-3,4,7,8-tetrahydropteridin-6-yl)methyl]amino]-N-(5-methylisoxazol-3-yl)benzenesulfonamide | - |
? | |
| sulfanilamide + 6-hydroxymethyl-7,8-dihydropterin diphosphate | - |
Bacillus anthracis | diphosphate + 4-[[(2-amino-4-oxo-3,4,7,8-tetrahydropteridin-6-yl)methyl]amino]benzenesulfonamide | - |
? | |
| sulfathiazole + 6-hydroxymethyl-7,8-dihydropterin diphosphate | - |
Bacillus anthracis | diphosphate + 4-[[(2-amino-4-oxo-3,4,7,8-tetrahydropteridin-6-yl)methyl]amino]-N-1,3-thiazol-2-ylbenzenesulfonamide | - |
? | |
| sulfisoxazole + 6-hydroxymethyl-7,8-dihydropterin diphosphate | - |
Bacillus anthracis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHPS | - |
Bacillus anthracis |
| dihydropteroate synthase | - |
Bacillus anthracis |
| FolP | - |
Bacillus anthracis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | linear dependency on DHPS concentration when concentration of enzyme versus initial velocity in the coupled enzymatic assay | Bacillus anthracis | |
| 0.0578 | - |
dapson | in absence of 4-aminobenzoate | Bacillus anthracis | |
| 0.079 | - |
sulfamethizole | in absence of 4-aminobenzoate | Bacillus anthracis | |
| 0.08 | - |
sulfanilamide | in absence of 4-aminobenzoate | Bacillus anthracis | |
| 0.0887 | - |
sulfathiazole | in absence of 4-aminobenzoate | Bacillus anthracis | |
| 0.109 | - |
4-Aminobenzoate | 100 microM DHP-PP, 25 °C | Bacillus anthracis | |
| 0.11 | - |
sulfisoxazole | in absence of 4-aminobenzoate | Bacillus anthracis | |
| 0.12 | - |
sulfamethoxazole | in absence of 4-aminobenzoate | Bacillus anthracis | |
| 0.1243 | - |
Sulfadiazine | in absence of 4-aminobenzoate | Bacillus anthracis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0036 | - |
6-hydroxymethylpterin monophosphate | 100 microM 4-aminobenzoate | Bacillus anthracis | |
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