Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | coupled enzymatic spectrophotometric assay to measure the activity of DHPS | Saccharomyces cerevisiae |
P559S | mutant with implicated sulfa drug resistance | Saccharomyces cerevisiae |
T557A | mutant with implicated sulfa drug resistance | Saccharomyces cerevisiae |
T557A/P559S | mutant with implicated sulfa drug resistance | Saccharomyces cerevisiae |
T557V/P559S | mutant with implicated sulfa drug resistance | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dapsone | - |
Saccharomyces cerevisiae | |
sulfachloropyridazine | - |
Saccharomyces cerevisiae | |
sulfamethoxazole | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
p-Aminobenzoic acid | wild type | Saccharomyces cerevisiae | |
0.0485 | - |
p-Aminobenzoic acid | mutant P559S | Saccharomyces cerevisiae | |
0.103 | - |
p-Aminobenzoic acid | mutant T557A | Saccharomyces cerevisiae | |
1.161 | - |
p-Aminobenzoic acid | mutant T557V/P559S | Saccharomyces cerevisiae | |
4.02 | - |
p-Aminobenzoic acid | mutant T557A/P559S | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate | Saccharomyces cerevisiae | - |
diphosphate + 7,8-dihydropteroate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P53848 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate | - |
Saccharomyces cerevisiae | diphosphate + 7,8-dihydropteroate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
7,8-dihydropteroate synthase | - |
Saccharomyces cerevisiae |
DHPS | - |
Saccharomyces cerevisiae |
dihydropteroate synthase | - |
Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00068 | - |
dapsone | wild tpye | Saccharomyces cerevisiae | |
0.00205 | - |
dapsone | mutant T557A | Saccharomyces cerevisiae | |
0.00295 | - |
sulfachloropyridazine | wild tpye | Saccharomyces cerevisiae | |
0.00371 | - |
sulfamethoxazole | mutant T557A | Saccharomyces cerevisiae | |
0.0063 | - |
sulfachloropyridazine | mutant T557A | Saccharomyces cerevisiae | |
0.0085 | - |
sulfamethoxazole | wild tpye | Saccharomyces cerevisiae | |
0.0148 | - |
dapsone | mutant P559S | Saccharomyces cerevisiae | |
0.0513 | - |
sulfachloropyridazine | mutant P559S | Saccharomyces cerevisiae | |
0.0543 | - |
sulfamethoxazole | mutant P559S | Saccharomyces cerevisiae | |
0.096 | - |
sulfachloropyridazine | mutant T557A/P559S | Saccharomyces cerevisiae | |
0.76 | - |
sulfamethoxazole | mutant T557V/P559S | Saccharomyces cerevisiae | |
0.94 | - |
sulfachloropyridazine | mutant T557V/P559S | Saccharomyces cerevisiae | |
1.16 | - |
dapsone | mutant T557A/P559S | Saccharomyces cerevisiae | |
1.17 | - |
sulfamethoxazole | mutant T557A/P559S | Saccharomyces cerevisiae | |
1.29 | - |
dapsone | mutant T557V/P559S | Saccharomyces cerevisiae |