Literature summary for 2.5.1.15 extracted from

Nopponpunth, V.; Sirawaraporn, W.; Greene, P.J.; Santi, D.V.
Cloning and expression of Mycobacterium tuberculosis and Mycobacterium leprae dihydropteroate synthase in Escherichia coli (1999), J. Bacteriol., 181, 6814-6821.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in dihydroxypteroate synthase deficient strain of Escherichia coli	Mycobacterium tuberculosis
expression in dihydroxypteroate synthase deficient strain of Escherichia coli	Mycobacterium leprae

Inhibitors

Inhibitors	Comment	Organism	Structure
dapsone	-	Mycobacterium leprae
dapsone	-	Mycobacterium tuberculosis
p-Aminosalicylate	-	Mycobacterium leprae
p-Aminosalicylate	-	Mycobacterium tuberculosis
sulfamethoxazole	-	Mycobacterium leprae
sulfamethoxazole	-	Mycobacterium tuberculosis
sulfamethoxypyridazine	-	Mycobacterium leprae
sulfamethoxypyridazine	-	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00037	-	p-Aminobenzoic acid	-	Mycobacterium tuberculosis
0.0006	-	p-Aminobenzoic acid	-	Mycobacterium leprae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	gel filtration of Mycobacterium tuberculosis and Mycobacterium leprae dihydropteroate synthase	Mycobacterium tuberculosis
60000	-	gel filtration of Mycobacterium tuberculosis and Mycobacterium leprae dihydropteroate synthase	Mycobacterium leprae

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium leprae	P0C0X1	-	-
Mycobacterium tuberculosis	P9WND1	-	-
Mycobacterium tuberculosis H37Rv	P9WND1	-	-

Purification (Commentary)

Purification (Comment)	Organism
mycobacterial enzymes, from DEAE-Sepharose and other chromatography steps	Mycobacterium tuberculosis
mycobacterial enzymes, from DEAE-Sepharose and other chromatography steps	Mycobacterium leprae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.174	-	recombinant enzyme	Mycobacterium leprae
0.596	-	recombinant enzyme	Mycobacterium tuberculosis

Storage Stability

Storage Stability	Organism
-80ºC, pH 7.0, 20% glycerol, 10-30% loss of activity in 1 month	Mycobacterium tuberculosis
-80ºC, pH 7.0, 20% glycerol, 10-30% loss of activity in 1 month	Mycobacterium leprae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate	-	Mycobacterium tuberculosis	diphosphate + 7,8-dihydropteroate	-	?
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate	-	Mycobacterium leprae	diphosphate + 7,8-dihydropteroate	-	?
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate	-	Mycobacterium tuberculosis H37Rv	diphosphate + 7,8-dihydropteroate	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Mycobacterium tuberculosis
dimer	-	Mycobacterium leprae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Mycobacterium leprae
9	-	-	Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000011	-	dapsone	-	Mycobacterium leprae
0.000013	-	dapsone	-	Mycobacterium tuberculosis
0.000025	-	sulfamethoxypyridazine	-	Mycobacterium leprae
0.000028	-	sulfamethoxazole	-	Mycobacterium tuberculosis
0.00003	-	sulfamethoxazole	-	Mycobacterium leprae
0.000031	-	sulfamethoxypyridazine	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)