Literature summary for 2.5.1.15 extracted from

Talarico, T.L.; Dev, I.K.; Dallas, W.S.; Ferone, R.; Ray, P.H.
Purification and partial characterization of 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase and 7,8-dihydropteroate synthase from Escherichia coli MC4100 (1991), J. Bacteriol., 173, 7029-7032.

Search for more literature for 2.5.1.15

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0005	-	4-Aminobenzoate	-	Escherichia coli MC4100
0.0019	-	2-Amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate	-	Escherichia coli MC4100

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Escherichia coli MC4100	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	2 * 30000, SDS-PAGE	Escherichia coli MC4100
52000	54000	gel filtration	Escherichia coli MC4100

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli MC4100	P0AC13	-	-

Purification (Commentary)

Purification (Comment)	Organism
precipitation with ammonium sulfate followed by column chromatography	Escherichia coli MC4100

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.7	-	-	Escherichia coli MC4100

Storage Stability

Storage Stability	Organism
-20°C or -70°C, 20 mM Tris/HCl, pH 8.0, 5 weeks	Escherichia coli MC4100

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate	-	Escherichia coli MC4100	diphosphate + 7,8-dihydropteroate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 30000, SDS-PAGE	Escherichia coli MC4100

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Escherichia coli MC4100

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)