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Literature summary for 2.5.1.15 extracted from

  • Roland, S.; Ferone, R.; Harvey, R.J.; Styles, V.L.; Morrison, R.W.
    The characteristics and significance of sulfonamides as substrates for Escherichia coli dihydropteroate synthase (1979), J. Biol. Chem., 254, 10337-10345.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
sulfamethoxazole competitive to 4-aminobenzoate Escherichia coli
sulfanilamide
-
Escherichia coli
sulfathiazole competitive to 4-aminobenzoate Escherichia coli
sulfonamide competitive to 4-aminobenzoate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00012
-
sulfamethoxazole
-
Escherichia coli
0.00057
-
4-Aminobenzoate
-
Escherichia coli
0.001
-
sulfathiazole
-
Escherichia coli
0.0048
-
sulfanilamide
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate Escherichia coli
-
diphosphate + 7,8-dihydropteroate
-
?
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate Escherichia coli B / ATCC 11303
-
diphosphate + 7,8-dihydropteroate
-
?
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + sulfamethoxazole Escherichia coli
-
diphosphate + dihydropterin-sulfamethoxazole
-
?
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + sulfamethoxazole Escherichia coli B / ATCC 11303
-
diphosphate + dihydropterin-sulfamethoxazole
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration on Sephadex G-100 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1 2
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
-
Escherichia coli diphosphate + 7,8-dihydropteroate
-
?
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
-
Escherichia coli B / ATCC 11303 diphosphate + 7,8-dihydropteroate
-
?
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + sulfamethoxazole
-
Escherichia coli diphosphate + dihydropterin-sulfamethoxazole
-
?
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + sulfamethoxazole
-
Escherichia coli B / ATCC 11303 diphosphate + dihydropterin-sulfamethoxazole
-
?
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + sulfanilamide
-
Escherichia coli diphosphate + dihydropterin-sulfanilamide
-
?
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + sulfathiazole
-
Escherichia coli diphosphate + dihydropterin-sulfathiazole
-
?
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + sulfathiazole
-
Escherichia coli B / ATCC 11303 diphosphate + dihydropterin-sulfathiazole
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00013
-
sulfamethoxazole
-
Escherichia coli
0.00053
-
sulfonamide
-
Escherichia coli
0.0057
-
sulfanilamide
-
Escherichia coli