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Literature summary for 2.5.1.10 extracted from

  • Ferriols, V.M.; Yaginuma, R.; Adachi, M.; Takada, K.; Matsunaga, S.; Okada, S.
    Cloning and characterization of farnesyl pyrophosphate synthase from the highly branched isoprenoid producing diatom Rhizosolenia setigera (2015), Sci. Rep., 5, 10246.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhizosolenia setigera

Inhibitors

Inhibitors Comment Organism Structure
risedronate
-
 Rhizosolenia setigera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0106
-
 dimethylallyl diphosphate at pH 7.5% and 30°C Rhizosolenia setigera
0.0212
-
 isopentenyl diphosphate with geranyl diphosphate as cosubstrate, at pH 7.5% and 30°C Rhizosolenia setigera
0.0215
-
 isopentenyl diphosphate with dimethylallyl diphosphate as cosubstrate, at pH 7.5% and 30°C Rhizosolenia setigera
0.063
-
 geranyl diphosphate at pH 7.5% and 30°C Rhizosolenia setigera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
-
 Rhizosolenia setigera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate + isopentenyl diphosphate Rhizosolenia setigera
-
 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
geranyl diphosphate + isopentenyl diphosphate Rhizosolenia setigera CCMP1694
-
 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
isopentenyl diphosphate + dimethylallyl diphosphate Rhizosolenia setigera
-
 diphosphate + geranyl diphosphate
-
 ?
isopentenyl diphosphate + dimethylallyl diphosphate Rhizosolenia setigera CCMP1694
-
 diphosphate + geranyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizosolenia setigera
-
-
-
Rhizosolenia setigera CCMP1694
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate + isopentenyl diphosphate
-
 Rhizosolenia setigera diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
geranyl diphosphate + isopentenyl diphosphate
-
 Rhizosolenia setigera CCMP1694 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
isopentenyl diphosphate + dimethylallyl diphosphate
-
 Rhizosolenia setigera diphosphate + geranyl diphosphate
-
 ?
isopentenyl diphosphate + dimethylallyl diphosphate
-
 Rhizosolenia setigera CCMP1694 diphosphate + geranyl diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 48940, calculated from amino acid sequence Rhizosolenia setigera
? x * 53000, recombinant His6-tagged enzyme, SDS-PAGE Rhizosolenia setigera

Synonyms

Synonyms Comment Organism
farnesyl pyrophosphate synthase
-
 Rhizosolenia setigera
FPPS
-
 Rhizosolenia setigera

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.73
-
 dimethylallyl diphosphate at pH 7.5% and 30°C Rhizosolenia setigera
6
-
 isopentenyl diphosphate with dimethylallyl diphosphate as cosubstrate, at pH 7.5% and 30°C Rhizosolenia setigera
33.5
-
 isopentenyl diphosphate with geranyl diphosphate as cosubstrate, at pH 7.5% and 30°C Rhizosolenia setigera
43.5
-
 geranyl diphosphate at pH 7.5% and 30°C Rhizosolenia setigera

General Information

General Information Comment Organism
metabolism the enzyme supplies precursors for highly branched isoprenoid biosynthesis Rhizosolenia setigera