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Literature summary for 2.5.1.10 extracted from
- Purification and characterization of two forms of geranyl transferase from Ricinus communis (1974), Biochemistry, 13, 4720-4729.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 8 mM, 33% inhibition | Ricinus communis |  |
| Mn2+ | activates at 0.25-0.50 mM, inhibition above | Ricinus communis | |
| additional information | neither iodoacetamide nor N-ethylmaleimide inhibits at concentration less than 5 mM | Ricinus communis | |
| N-ethylmaleimide | 8 mM, 54% inhibition | Ricinus communis | |
| p-hydroxymercuribenzoate | 1 mM, 25% inhibition | Ricinus communis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0045 | - |
dimethylallyl diphosphate | 0.042-0.056 mg/ml protein, isoenzyme II, Km values depend on protein concentration | Ricinus communis | |
| 0.0059 | - |
dimethylallyl diphosphate | 0.042-0.056 mg/ml protein, isoenzyme I, Km values depend on protein concentration | Ricinus communis | |
| 0.032 | - |
isopentenyl diphosphate | 0.042-0.056 mg/ml protein, isoenzyme II, Km values depend on protein concentration | Ricinus communis | |
| 0.036 | - |
geranyl diphosphate | 0.042-0.056 mg/ml protein, isoenzyme I, Km values depend on protein concentration | Ricinus communis | |
| 0.039 | - |
geranyl diphosphate | 0.042-0.056 mg/ml protein, isoenzyme II, Km values depend on protein concentration | Ricinus communis | |
| 0.048 | - |
isopentenyl diphosphate | 0.042-0.056 mg/ml protein, isoenzyme I, Km values depend on protein concentration | Ricinus communis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Ricinus communis | |
| Mg2+ | maximum activity at 1-2 mM | Ricinus communis | |
| Mn2+ | activation at 0.25-0.50 mM, inhibition above | Ricinus communis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
gel filtration, enzyme I, protein concentration 0.02 mg/ml | Ricinus communis |
| 60000 | - |
gel filtration, enzyme II, protein concentration 0.02 mg/ml | Ricinus communis |
| 72500 | - |
gel filtration, protein concentration 25 mg/ml | Ricinus communis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
isoenzyme I and II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ricinus communis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.04 | - |
isoenzyme II | Ricinus communis |
| 0.06 | - |
isoenzyme I | Ricinus communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + 2 isopentenyl diphosphate | - |
Ricinus communis | (E,E)-farnesyl diphosphate + 2 diphosphate | - |
? | |
| geranyl diphosphate + isopentenyl diphosphate | - |
Ricinus communis | diphosphate + farnesyl diphosphate | E)-farnesyl diphosphate | ? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Ricinus communis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | approx. 45% of maximal activity at pH 5.5, approx. 60% of activity maximum at pH 8.5, emzyme I | Ricinus communis |
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