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Literature summary for 2.4.2.8 extracted from

  • Bowen, T.L.; Lin, W.C.; Whitman, W.B.
    Characterization of guanine and hypoxanthine phosphoribosyltransferases in Methanococcus voltae (1996), J. Bacteriol., 178, 2521-2526.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
GMP 0.05 mM, at least 2fold stimulation, hypoxanthine phosphoribosyltransferase activity Methanococcus voltae
IMP 0.2 mM, at least 2fold stimulation, hypoxanthine phosphoribosyltransferase activity Methanococcus voltae
additional information hypoxanthine phosphoribosyltransferase activity is unaffected by AMP Methanococcus voltae

Inhibitors

Inhibitors Comment Organism Structure
GMP 0.3 mM, 50% inhibition of guanine phosphoribosyltransferase activity Methanococcus voltae
guanine 0.06 mM, 50% inhibition of hypoxanthine phosphoribosyltransferase activity Methanococcus voltae
hypoxanthine 0.16 mM, 50% inhibition of guanine phosphoribosyltransferase activity Methanococcus voltae
additional information guanine phosphoribosyltransferase activity: IMP and AMP are not inhibitory at concentrations up to 0.6 mM, adenine and xanthine are not inhibitory at concentrations up to 0.5 mM. Adenine and xanthine do not inhibit hypoxanthine phosphoribosyltransferase activity Methanococcus voltae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
hypoxanthine pH 9.0, 37°C Methanococcus voltae
0.2
-
guanine pH 9.0, 37°C Methanococcus voltae

Organism

Organism UniProt Comment Textmining
Methanococcus voltae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00085
-
pH 9.0, 37°C, hypoxanthine phosphoribosyltransferase activity Methanococcus voltae
0.0027
-
pH 9.0, 37°C, guanine phosphoribosyltransferase activity Methanococcus voltae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Methanococcus voltae GMP + diphosphate
-
?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Methanococcus voltae IMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
guanine PRTase
-
Methanococcus voltae
hypoxanthine PRTase
-
Methanococcus voltae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
guanine phosphoribosyltransferase activity and hypoxanthine phosphoribosyltransferase activity Methanococcus voltae

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 half-maximal activity at pH 7.5, and 10.0, guanine phosphoribosyltransferase activity Methanococcus voltae
7.5 9.5 half-maximal activity at pH 7.5, and 9.5, hypoxanthine phosphoribosyltransferase activity Methanococcus voltae