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Literature summary for 2.4.2.8 extracted from

  • Raman, J.; Ashok, C.S.; Subbayya, S.I.; Anand, R.P.; Selvi, S.T.; Balaram, H.
    Plasmodium falciparum hypoxanthine guanine phosphoribosyltransferase. Stability studies on the product-activated enzyme (2005), FEBS J., 272, 1900-1911.
    View publication on PubMed
Search for more literature for 2.4.2.8

Activating Compound

Activating Compound Comment Organism Structure
additional information activation by substrate and product IMP, reversibly destabilizes the enzyme Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzyme in Escherichia coli, complementation of an enzyme-deficient Escherichia coli strain by the wild-type and mutant L44F at growth temperatures 20°C and 37°C, and at 42°C only by the wild-type enzyme Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
L44F site-directed mutagenesis, temperature-sensitive mutant, no complementation of an enzyme-deficient Escherichia coli strain at 42°C, only at 20°C and 37°C Plasmodium falciparum

General Stability

General Stability Organism
activation by substrate and product IMP destabilizes the enzyme, the unactivated ligand-free enzyme is more stable Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum
-
 GMP + diphosphate
-
 r
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum
-
 IMP + diphosphate
-
 r
additional information Plasmodium falciparum the enzyme activity is regulated by stabilization and destabilization of the enzyme through release or binding of product and substrates, overview ?
-
 ?
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum low activity xanthosine 5'-phosphate + diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant L44F from Escherichia coli, the enzyme shows very low activity Plasmodium falciparum

Reaction

Reaction Comment Organism Reaction ID
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate ordered bi bi kinetic mechanism with 5-phospho-alpha-D-ribose 1-diphosphate binding first followed by the purine base Plasmodium falciparum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.45
-
 purified recombinant enzyme, substrate hypoxanthine, in presence of 0.008 mM IMP, after 6 h Plasmodium falciparum
5.22
-
 purified recombinant enzyme, substrate hypoxanthine, in presence of 0.038 mM IMP, after 48 h Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Plasmodium falciparum GMP + diphosphate
-
 r
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Plasmodium falciparum IMP + diphosphate
-
 r
additional information the enzyme activity is regulated by stabilization and destabilization of the enzyme through release or binding of product and substrates, overview Plasmodium falciparum ?
-
 ?
additional information the purified recombinant enzyme shows very low activity Plasmodium falciparum ?
-
 ?
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate low activity Plasmodium falciparum xanthosine 5'-phosphate + diphosphate
-
 r

Synonyms

Synonyms Comment Organism
HGPRT
-
 Plasmodium falciparum
hypoxanthine guanine phosphoribosyltransferase
-
 Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
-
 Plasmodium falciparum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
-
 Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Plasmodium falciparum