Any feedback?
Literature summary for 2.4.2.8 extracted from
- Unusual substrate specificity of a chimeric hypoxanthine-guanine phosphoribosyltransferase containing segments from the Plasmodium falciparum and human enzymes (2000), Biochem. Biophys. Res. Commun., 272, 596-602.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and chimeric enzymes in enzyme-deficient Escherichia coli strain, complementation study | Homo sapiens |
| expression of wild-type and chimeric enzymes in enzyme-deficient Escherichia coli strain, complementation study, overexpression of chimeric mutant DS1 | Plasmodium falciparum |
| overexpression in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of 4 chimeric enzymes with segments of human and Plasmodium falciparum enzymes, altered substrate specificities | Homo sapiens |
| additional information | construction of 4 chimeric enzymes with segments of human and Plasmodium falciparum enzymes, altered substrate specificities | Plasmodium falciparum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
hypoxanthine | wild-type enzyme | Homo sapiens |  |
| 0.0011 | - |
guanine | recombinant chimeric mutant DS1 | Homo sapiens | |
| 0.0011 | - |
guanine | recombinant chimeric mutant DS1 | Plasmodium falciparum | |
| 0.0014 | - |
hypoxanthine | recombinant chimeric mutant DS1 | Homo sapiens | |
| 0.0014 | - |
hypoxanthine | recombinant chimeric mutant DS1 | Plasmodium falciparum | |
| 0.005 | - |
guanine | wild-type enzyme | Homo sapiens | |
| 0.3 | - |
xanthine | above, purified recombinant chimeric enzyme DS1 | Homo sapiens | |
| 0.3 | - |
xanthine | above, purified recombinant chimeric enzyme DS1 | Plasmodium falciparum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
| Mg2+ | - |
Plasmodium falciparum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
construction of 4 chimeric enzymes with segments of human and Plasmodium falciparum enzymes | - |
| Plasmodium falciparum | - |
construction of 4 chimeric enzymes with segments of human and Plasmodium falciparum enzymes | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.032 | - |
purified recombinant chimeric enzyme DS1, substrate guanine | Homo sapiens |
| 0.032 | - |
purified recombinant chimeric enzyme DS1, substrate guanine | Plasmodium falciparum |
| 0.147 | - |
purified recombinant chimeric enzyme DS1, substrate xanthine | Homo sapiens |
| 0.147 | - |
purified recombinant chimeric enzyme DS1, substrate xanthine | Plasmodium falciparum |
| 0.226 | - |
purified recombinant chimeric enzyme DS1, substrate hypoxanthine | Homo sapiens |
| 0.226 | - |
purified recombinant chimeric enzyme DS1, substrate hypoxanthine | Plasmodium falciparum |
| 11.9 | - |
purified recombinant enzyme, substrate hypoxanthine | Homo sapiens |
| 25.8 | - |
purified recombinant enzyme, substrate guanine | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | GMP + diphosphate | - |
? | |
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | GMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | IMP + diphosphate | - |
? | |
| xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | no activity | Homo sapiens | XMP + diphosphate | - |
? | |
| xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | recombinant chimeric mutant DS1 | Homo sapiens | XMP + diphosphate | - |
? | |
| xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | recombinant chimeric mutant DS1 | Plasmodium falciparum | XMP + diphosphate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
guanine | purified recombinant chimeric enzyme DS1 | Homo sapiens | |
| 0.013 | - |
guanine | purified recombinant chimeric enzyme DS1 | Plasmodium falciparum | |
| 0.062 | - |
xanthine | purified recombinant chimeric enzyme DS1 | Homo sapiens | |
| 0.062 | - |
xanthine | purified recombinant chimeric enzyme DS1 | Plasmodium falciparum | |
| 0.073 | - |
hypoxanthine | purified recombinant chimeric enzyme DS1 | Homo sapiens | |
| 0.073 | - |
hypoxanthine | purified recombinant chimeric enzyme DS1 | Plasmodium falciparum | |
| 5.7 | - |
hypoxanthine | wild-type enzyme | Homo sapiens | |
| 10.5 | - |
guanine | wild-type enzyme | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
| 7.4 | - |
assay at | Plasmodium falciparum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
