Cloned (Comment) | Organism |
---|---|
functional expression of wild-type enzyme and mutants in an enzyme-deficient Escherichia coli strain | Leishmania donovani |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutant lacking the Ser-Lys-Val C-terminal targeting signal, mutant enzyme is located throughout the parasite, including subcellular organelles such as nucleus and flagellum | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
hypoxanthine | mutant enzyme | Leishmania donovani | |
0.0064 | - |
hypoxanthine | wild-type enzyme | Leishmania donovani | |
0.01 | - |
guanine | wild-type enzyme | Leishmania donovani | |
0.012 | - |
guanine | mutant enzyme | Leishmania donovani |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | a fuel-metabolizing microbody unique to this parasite, exclusively | Leishmania donovani | 20015 | - |
additional information | the mutant enzyme, lacking the targeting signal, is located throughout the parasite, including subcellular organelles such as nucleus and flagellum | Leishmania donovani | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Leishmania donovani | - |
GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Leishmania donovani | - |
IMP + diphosphate | - |
? | |
additional information | Leishmania donovani | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
parasites isolated from rabbits | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant from Escherichia coli | Leishmania donovani |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
promastigote | - |
Leishmania donovani | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | GMP + diphosphate | - |
? | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | GMP + diphosphate | - |
r | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | IMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | IMP + diphosphate | - |
r | |
additional information | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | Leishmania donovani | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.7 | - |
hypoxanthine | wild-type enzyme | Leishmania donovani | |
6.2 | - |
hypoxanthine | mutant enzyme | Leishmania donovani | |
12.1 | - |
guanine | wild-type enzyme | Leishmania donovani | |
15.8 | - |
guanine | mutant enzyme | Leishmania donovani |