Any feedback?
Literature summary for 2.4.2.4 extracted from
- The kinetic mechanism of human thymidine phosphorylase - a molecular target for cancer drug development (2014), Mol. Biosyst., 10, 592-604.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta (DE3) cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KIN59 | i.e. 5'-O-tritylinosine, noncompetitive inhibition | Homo sapiens |  |
| thymidine | substrate inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.066 | - |
thymidine | at pH 7.5 at 37°C | Homo sapiens | |
| 0.107 | - |
phosphate | at pH 7.5 at 37°C | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48855 | - |
2 * 48855, mass spectrometry | Homo sapiens |
| 49024 | - |
2 * 49024, calculated from amino acid sequence | Homo sapiens |
| 50000 | - |
2 * 50000, SDS-PAGE | Homo sapiens |
| 121000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thymidine + phosphate | Homo sapiens | - |
thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P19971 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose column chromatography and phenyl Sepharose column chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.24 | - |
crude extract, at pH 7.5 at 37°C | Homo sapiens |
| 4.3 | - |
after 17.9fold purification, at pH 7.5 at 37°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thymidine + phosphate | - |
Homo sapiens | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 50000, SDS-PAGE | Homo sapiens |
| homodimer | 2 * 48855, mass spectrometry | Homo sapiens |
| homodimer | 2 * 49024, calculated from amino acid sequence | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gliostatin | - |
Homo sapiens |
| PD-ECGF | - |
Homo sapiens |
| platelet derived-endothelial cell growth factor | - |
Homo sapiens |
| TYMP | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.2 | - |
thymidine | at pH 7.5 at 37°C | Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | thymidine phosphorlyase is a key enzyme in the pyrimidine salvage pathway and involved in thymidine homeostasis in cells | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 67 | - |
phosphate | at pH 7.5 at 37°C | Homo sapiens | |
| 109 | - |
thymidine | at pH 7.5 at 37°C | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
