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Literature summary for 2.4.2.4 extracted from

  • Avraham, Y.; Yashphe, J.; Grossowicz, N.
    Thymidine phosphorylase and uridine phosphorylase of Lactobacillus casei (1988), FEMS Microbiol. Lett., 56, 29-34.
No PubMed abstract available
Search for more literature for 2.4.2.4

Inhibitors

Inhibitors Comment Organism Structure
5-bromo-2-deoxyuridine 100% inhibition at 10 mM Lacticaseibacillus casei
5-fluorodeoxyuridine 80% inhibition at 10 mM Lacticaseibacillus casei
6-Methyluracil competitive, 86% inhibition at 10 mM and 36% inhibition at 1 mM Lacticaseibacillus casei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00166
-
 thymidine
-
 Lacticaseibacillus casei
0.1
-
 phosphate
-
 Lacticaseibacillus casei

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lacticaseibacillus casei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
111
-
-
 Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thymidine + phosphate
-
 Lacticaseibacillus casei thymine + 2-deoxy-D-ribose 1-phosphate
-
 r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 40 min, 10% loss of activity Lacticaseibacillus casei
60
-
 20 min, complete loss of activity Lacticaseibacillus casei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Lacticaseibacillus casei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6
-
 6-Methyluracil
-
 Lacticaseibacillus casei